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  • Electronic Resource  (10)
  • Cell & Developmental Biology  (10)
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  • Electronic Resource  (10)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 154 (1977), S. 133-145 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The junction between human primary dentine and regular and irregular secondary dentine was examined with a number of different light and electron microscopic techniques. In decalcified material, a narrow band along the innermost surface of the primary dentine stained intensely. The walls of the tubules within the band stained intensely, whereas the tubular walls within the bulk of the primary dentine were not stained. Generally, the walls of the tubules in both types of secondary dentine were also preferentially stained. Although not readily apparent in ground sections, observations of thin sections revealed a dramatic reduction in the number of tubules in regular secondary dentine. Generally, the radiodensity of the intertubular matrix was the same in primary and secondary dentine and the intensely stained band was not seen radiographically. The pulpal ends of the tubules in primary dentine were often occluded with a material having the same radiodensity as peritubular matrix. Both patent and occluded tubules were seen in irregular secondary dentine. Scanning electron microscopy of acid-etched specimens of secondary dentine revealed that some tubules had irregular walls of highly mineralized matrix which was less acid-soluble than the peritubular matrix of primary dentine.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 126 (1968), S. 435-445 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Thin semi-serial ground sections of coronal dentin were examined radiographically. The bulk of the coronal dentin was characterized by the majority of the tubules having a distinct peritubular zone. With the exception of the tubules running from the tip of the cusp to the pulp cornu, the bulk of peritubular matrix forming the walls of the tubules was disposed eccentrically. The matrix was thicker on the cervical sides of the tubule than it was on the incisal sides. In a relatively narrow layer of the coronal dentin between the bulk of the dentin and the predentindentin border area the thickness of the peritubular matrix varied considerably. It was extremely narrow or absent in some tubules and reached its greatest thickness in others. The tubules in the predentin border area showed little or no evidence of peritubular matrix. The area of dentin beneath the central developmental groove differed somewhat from the bulk of the dentin. Many of the tubules at all levels of this area showed little radiographic evidence of peritubular matrix. Obliterated tubules were seen in some of the sections taken immediately above the predentin-dentin border area in the region of the pulp cornu and were always seen at the junction of the mantle dentin and the circumpulpal dentin beneath the central developmental groove.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 141 (1973), S. 479-489 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: To determine the prism sheath configurations in human cuspal enamel 80 teeth were initially ground to produce flat surfaces through the following planes: a horizontal series at successively greater distances from the dentinoenamel junction and longitudinally through the center of the cusps. Individual teeth were suspended in an acid-alcohol solution (1 cm3 conc. HCl in 100 cm3 95% ethanol) at 37°C for seven to ten days. The treatment “softened” the enamel to a depth of approximately 1 mm. The teeth were embedded in Epon and sectioned at 0.5 to 10 μm with a diamond knife. Thick and thin ground sections for phase contrast microscopy and acid-etched ground sections for Nomarski differential interference microscopy were prepared through the same regions. In thicker longitudinal sections, the prisms in gnarled enamel formed a zig-zag pattern which was unlike the twisting pattern generally observed in ground sections. The thinnest transverse sections showed the sheath outlines to be dramatically different from those seen elsewhere in the enamel. Some prism sheaths were circular, others were in the form of spirals. What could be described as sheaths within sheaths were also seen. In the thinnest longitudinal sections the prisms were seen to be elongated and discontinuous. Sheath outlines in enamel adjacent to the central core of gnarled enamel were similar to those described elsewhere in the body of the enamel. Keyhole, modified keyhole patterns and arcade forms were the dominant sheath patterns. Other atypical sheath configurations were seen scattered throughout this region.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 563-569 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Human third molars were partially demineralized in an acid-alcohol solution and embedded in Epon 812. Six-micron sections were cut from regions of the cervical enamel exhibiting prominent Retzius lines. The plastic was removed from the specimens by microincineration and were examined with the scanning electron microscope. The most prominent structural feature seen along the Retzius lines was the cervical translocation of some of the prisms. The scanning electron microscopic images also suggested that prisms were translocating in the transverse plane of the tooth. A series of pores, which appeared to be empty, were observed in association with the translocations occurring along the Retzius lines.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 212 (1985), S. 336-344 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscopic (SEM) cast method that has been utilized to examine the internal structure of dentine was modified to examine canalicular communications in the cortices of a human femur and ulna. Although some preparations in which all of the matrix was removed were examined, etched preparations were found to be the most informative. Casts of lacunae and canaliculi along with the underlying matrix could be visualized in these preparations. In the femur, whose cortex exhibited a typical lamellar pattern, canalicular communication was seen between first and second generation osteons and occasionally between osteons and interstitial regions. The interstitial regions in the ulna appeared to be primarily woven rather than lamellar bone. Extensive communication between the outermost lacunae of osteons and interstitial regions was observed in the ulna.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 211 (1985), S. 149-155 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscope cast technique was used to examine the interface between primary and secondary dentine in young and old human teeth. In addition, more traditional methods were used to examine this interface with light microscopy, identical regions being viewed before and after demineralization. No continuity was seen between the tubules in primary dentine and those in irregular secondary dentine in the scanning electron microscope preparations. These preparations did show the tubules to be continuous between primary dentine and regular secondary dentine in young and old teeth. Both the scanning electron microscopic and light microscopic observations suggested that regular secondary dentine becomes highly sclerosed in old teeth.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 222 (1988), S. 121-127 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscopic cast technique was used to determine the nature of the canalicular system in human cellular cementum. Prior light microscopic observations suggested the presence of two distinct types of lacunae: bonelike and a large irregular type generally confined to the interradicular region. Only the bone-like lacunae were visualized in the SEM cast preparations. The canalicular system associated with the bone-like lacunae was usually continuous from the surface of the dentin to the surface of the cementum in newly-erupted teeth. Casts having a sponge-like configuration were observed near the cementodentinal junction in some of the specimens from the interradicular region. The presence of these casts could not be predicted from prior light microscopic observations and it was concluded that they may represent infiltration of hypomineralized matrix rather than lacunae.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 132 (1971), S. 375-391 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The neonatal line in developing human primary teeth was examined by means of phase contrast microscopy, microradiography and transmission electron microscopy. When thin sections were observed by light microscopy, the lateral dimensions of the line were not as extensive as had been previously reported. The line had a “staircase” configuration and appeared to be identical to published light micrographs of the stria of Retzius. On radiograms, the lateral extent of the hypomineralization was also decreased. The ultrastructural basis for the neonatal line appeared to be a localized change in configuration of enamel prisms along with a possible reduction in crystal concentration. The possibility that some rods actually end at the line could not be excluded, however.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 144 (1975), S. 407-419 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Both mature and developing human enamel were studied by correlative light and electron microscopic techniques. Ordinary light microscopic observations of routinely prepared ground sections produced a totally inaccurate impression of enamel prism direction. In contrast, ground sections which were acid-etched and viewed with phase contrast optics, as well as thin (0.5 μm) sections, were in concert with further electron microscopic preparations. The appearance of longitudinally sectioned prisms as demonstrated on thick ground sections is often the result of structural interrelation between groups of obliquely sectioned prisms. The arrangement between enamel prism heads and tails is often such as to produce the light microscopic appearance of classical enamel cross striations. The production of such optical artifacts may be related to conflicting interpretations concerning enamel cross striations as well as light microscopic descriptions of the histopathology of enamel caries.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 141 (1974), S. 91-103 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Unaltered mature enamel and partially demineralized mature enamel from the cervical third of human permanent teeth were examined by means of transmission electron microscopy, microradiography, phase contrast and Nomarski differential interference microscopy. The Retzius lines in the outer third of the cervical enamel had a staircase configuration. The steps in the stair-case pattern formed the bases of triangular regions which were radiolucent and ultrastructurally contained fewer crystallites than the adjacent enamel. In the outer prismless layer, the Retzius lines had a curvilinear configuration and were also crystallite deficient. The structure of the Retzius lines in the inner portion of the cervical enamel was not nearly as well defined.
    Type of Medium: Electronic Resource
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