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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 4 (1969), S. 257-259 
    ISSN: 1432-0827
    Keywords: Teeth ; Dentin ; Tubules ; Sclerosis ; Microradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Une méthode systématique de comptage par points a été utilisée pour déterminer le volume respectif de la dentine humaine coronaire péricanaliculaire et intercanaliculaire. Contrairement à l'opinion classique, il apparait que la dentine péricanaliculaire constitute une partie relativement importante de la dentine humaine coronaire, surtout au niveau de la moitié interne de la dentine. Dans cette couche, les proportions relatives des deux constitutants sont sensiblement égales.
    Abstract: Zusammenfassung Einc systematische Punktzählmethode wurde angewendet, um die Fraktionen der peritubulären Matrix im menschlichen Dentin der Zahnkrone volumetrisch auszuwerten. Entgegen der früheren Annahme zeigte es sich, daß das peritubuläre Dentin eine relativ große Fraktion des Dentins der Zahnkrone ausmacht; dies war besonders in der inneren Hälfte vom Dentin der Fall, wo die relativen Volumen der beiden Matrices ungefähr gleich groß waren.
    Notes: Abstract A systematic point-count method was utilized to determine the volume fractions of peritubular and intertubular matrix in human coronal dentin. Contrary to previous thought, it was found that peritubular dentin represents a relatively large fraction of human coronal dentin, particularly in the inner one-half of the dentin, where the relative volumes of the two matrices were approximately equal.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 8 (1971), S. 17-23 
    ISSN: 1432-0827
    Keywords: Dentin ; Matrix ; Microradiography ; Staining ; Decalcification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Un certain nombre de méthodes ont été décrites pour colorer de façon différente la matrice péricanaliculaire sur des coupes par usure de dentine humaine. Ces méthodes opèrent toutes à des pH qui sont susceptibles de décalcifier cette matrice. Des microradiographies de coupes par usure ont été réalisées avant et après coloration ou précoloration, ayant permis d'identifier la matrice péricanaliculaire. Les radiographies ont montré que cette matice est décalcifiée avant et à la fin du traitement. De telles techniques de coloration doivent être remplacées par des méthodes plus adaptées.
    Abstract: Zusammenfassung Verschiedene Forscher haben eine Anzahl von Methoden mitgeteilt, um die peritubuläre Matrix unentkalkter Schliffe von menschlichem Dentin differenziert zu färben. Allerdings wurden sämtliche dieser Methoden bei pH-Werten ausgeführt, die so hoch sind, daß die Möglichkeit besteht, die sehr säurelösliche peritubuläre Matrix zu entkalken. Von Schliffen wurden Mikroradiographien hergestellt, und zwar vor und nach den verschiedenen Färbeprozessen, welche nachgewiesenermaßen erfolgreich zum Färben von peritubulärer Matrix angewandt worden sind. Die Radiographien zeigten, daß die peritubuläre Matrix entweder am Schluß der vorgeschriebenen Behandlung oder sogar schon längst vorher entkalkt war. Es wurde daraus geschlossen, daß diese Färbetechniken durch neuere Methoden ersetzt werden sollten.
    Notes: Abstract Previous investigators have reported a number of methods which will differentially stain peritubular matrix in undecalcified ground sections of human dentin. The methods, however, were all carried out at pH values which were potentially high enough to decalcify the highly acid-soluble peritubular matrix. Microradiograms were prepared of ground sections both before and after they had been exposed to either the staining or pre-staining treatments which reportedly had been successful for staining peritubular matrix. The radiograms revealed that the peritubular matrix was decalcified either at the end of, or long before, the prescribed treatment had been completed. It was concluded that the use of these staining techniques should be re-evaluated by more recent methods.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 1 (1967), S. 319-323 
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des microradiographies de coupes minces minéralogiques de canalicules dentinaires, en coupe transversale, sont comparées a vec les aspects de ces mêmes canalicules en microscopie optique. Les anneaux clairs, visibles autour des canalicules en microscopie à lumière transparentc, en effectuant la mise au point sur la partie supérieure de la coupe, ne peuvent être mis en parallèle avec la zone péricanaliculaire — ces anneaux sont visibles dans la dentine d'incisive de rat, qui ne contient pas de matrice péricanaliculaire. Il semble donc que des coupes par usure, observée en lumière transparente classique, ne permettent pas d'identifier la zone péricanaliculaire.
    Abstract: Zusammenfassung Röntgenmikrographien von quergetroffenen Zahnbeinkanälchen in Schliffschnitten wurden mit gewöhnlichen lichtmikroskopischen Bildern verglichen. Die hellen Ringe, welche im gewöhnlichen lichtmikroskopischen Bild erscheinen, wenn das Mikroskop über die obere Schnittfläche eingestellt wird, konnten nicht mit einer röntgenmikrographisch dargestellten peritubulären Zone verglichen werden. Diese Ringe konnten im Zahnbein von Rattenschneidezähnen, die keine peritubuläre Zone besitzen, dargestellt werden. Es wurde festgestellt, daß die im gewöhnlichen Licht beobachteten Schliffschnitte nicht für die Herstellung der peritubulären Zonen geeignet sind.
    Notes: Abstract Microradiograms of thin ground sections of transversely cut dentinal tubules were compared to the light microscopic images of the ground sections. The bright rings seen around tubules viewed by ordinary transmitted light with the objective focused on the upper surface of the section could not be related to a radiographically demonstrable peritobular zone. These rings could be produced in rat incisor dentin which does not have peritubular matrix. It was concluded that ground sections viewed with ordinarily transmitted light could not be validly used to determine the presence of peritubular matrix.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 222 (1988), S. 121-127 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscopic cast technique was used to determine the nature of the canalicular system in human cellular cementum. Prior light microscopic observations suggested the presence of two distinct types of lacunae: bonelike and a large irregular type generally confined to the interradicular region. Only the bone-like lacunae were visualized in the SEM cast preparations. The canalicular system associated with the bone-like lacunae was usually continuous from the surface of the dentin to the surface of the cementum in newly-erupted teeth. Casts having a sponge-like configuration were observed near the cementodentinal junction in some of the specimens from the interradicular region. The presence of these casts could not be predicted from prior light microscopic observations and it was concluded that they may represent infiltration of hypomineralized matrix rather than lacunae.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 563-569 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Human third molars were partially demineralized in an acid-alcohol solution and embedded in Epon 812. Six-micron sections were cut from regions of the cervical enamel exhibiting prominent Retzius lines. The plastic was removed from the specimens by microincineration and were examined with the scanning electron microscope. The most prominent structural feature seen along the Retzius lines was the cervical translocation of some of the prisms. The scanning electron microscopic images also suggested that prisms were translocating in the transverse plane of the tooth. A series of pores, which appeared to be empty, were observed in association with the translocations occurring along the Retzius lines.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 211 (1985), S. 149-155 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscope cast technique was used to examine the interface between primary and secondary dentine in young and old human teeth. In addition, more traditional methods were used to examine this interface with light microscopy, identical regions being viewed before and after demineralization. No continuity was seen between the tubules in primary dentine and those in irregular secondary dentine in the scanning electron microscope preparations. These preparations did show the tubules to be continuous between primary dentine and regular secondary dentine in young and old teeth. Both the scanning electron microscopic and light microscopic observations suggested that regular secondary dentine becomes highly sclerosed in old teeth.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 212 (1985), S. 336-344 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A scanning electron microscopic (SEM) cast method that has been utilized to examine the internal structure of dentine was modified to examine canalicular communications in the cortices of a human femur and ulna. Although some preparations in which all of the matrix was removed were examined, etched preparations were found to be the most informative. Casts of lacunae and canaliculi along with the underlying matrix could be visualized in these preparations. In the femur, whose cortex exhibited a typical lamellar pattern, canalicular communication was seen between first and second generation osteons and occasionally between osteons and interstitial regions. The interstitial regions in the ulna appeared to be primarily woven rather than lamellar bone. Extensive communication between the outermost lacunae of osteons and interstitial regions was observed in the ulna.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 126 (1968), S. 435-445 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Thin semi-serial ground sections of coronal dentin were examined radiographically. The bulk of the coronal dentin was characterized by the majority of the tubules having a distinct peritubular zone. With the exception of the tubules running from the tip of the cusp to the pulp cornu, the bulk of peritubular matrix forming the walls of the tubules was disposed eccentrically. The matrix was thicker on the cervical sides of the tubule than it was on the incisal sides. In a relatively narrow layer of the coronal dentin between the bulk of the dentin and the predentindentin border area the thickness of the peritubular matrix varied considerably. It was extremely narrow or absent in some tubules and reached its greatest thickness in others. The tubules in the predentin border area showed little or no evidence of peritubular matrix. The area of dentin beneath the central developmental groove differed somewhat from the bulk of the dentin. Many of the tubules at all levels of this area showed little radiographic evidence of peritubular matrix. Obliterated tubules were seen in some of the sections taken immediately above the predentin-dentin border area in the region of the pulp cornu and were always seen at the junction of the mantle dentin and the circumpulpal dentin beneath the central developmental groove.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 154 (1977), S. 133-145 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The junction between human primary dentine and regular and irregular secondary dentine was examined with a number of different light and electron microscopic techniques. In decalcified material, a narrow band along the innermost surface of the primary dentine stained intensely. The walls of the tubules within the band stained intensely, whereas the tubular walls within the bulk of the primary dentine were not stained. Generally, the walls of the tubules in both types of secondary dentine were also preferentially stained. Although not readily apparent in ground sections, observations of thin sections revealed a dramatic reduction in the number of tubules in regular secondary dentine. Generally, the radiodensity of the intertubular matrix was the same in primary and secondary dentine and the intensely stained band was not seen radiographically. The pulpal ends of the tubules in primary dentine were often occluded with a material having the same radiodensity as peritubular matrix. Both patent and occluded tubules were seen in irregular secondary dentine. Scanning electron microscopy of acid-etched specimens of secondary dentine revealed that some tubules had irregular walls of highly mineralized matrix which was less acid-soluble than the peritubular matrix of primary dentine.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 141 (1973), S. 479-489 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: To determine the prism sheath configurations in human cuspal enamel 80 teeth were initially ground to produce flat surfaces through the following planes: a horizontal series at successively greater distances from the dentinoenamel junction and longitudinally through the center of the cusps. Individual teeth were suspended in an acid-alcohol solution (1 cm3 conc. HCl in 100 cm3 95% ethanol) at 37°C for seven to ten days. The treatment “softened” the enamel to a depth of approximately 1 mm. The teeth were embedded in Epon and sectioned at 0.5 to 10 μm with a diamond knife. Thick and thin ground sections for phase contrast microscopy and acid-etched ground sections for Nomarski differential interference microscopy were prepared through the same regions. In thicker longitudinal sections, the prisms in gnarled enamel formed a zig-zag pattern which was unlike the twisting pattern generally observed in ground sections. The thinnest transverse sections showed the sheath outlines to be dramatically different from those seen elsewhere in the enamel. Some prism sheaths were circular, others were in the form of spirals. What could be described as sheaths within sheaths were also seen. In the thinnest longitudinal sections the prisms were seen to be elongated and discontinuous. Sheath outlines in enamel adjacent to the central core of gnarled enamel were similar to those described elsewhere in the body of the enamel. Keyhole, modified keyhole patterns and arcade forms were the dominant sheath patterns. Other atypical sheath configurations were seen scattered throughout this region.
    Type of Medium: Electronic Resource
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