ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The construction and performance of new carbon paste enzyme electrodes as amperometric sensors is described. Xanthine oxidase and alkaline phosphatase have been adsorbed on a carbon paste electrode (CPE), and physically entrapped with a semipermeable membrane. The product of enzymatic reaction, uric acid or p-nitrophenol, was electrochemically oxidized versus an Ag/AgCl electrode at +0.4 or +0.8 V, respectively, yielding a steady-state current directly related to the substrate concentration. Response characteristics as well as kinetic parameters have been evaluated.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140030412
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