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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of new quinidine metabolites and simultaneous determination of quinidine and its metabolites in blood and urine by direct injection HPLC analysis (1985)
    Springer
    Chromatographia 20 (1985), S. 671-676 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Direct injection of biological samples ; New quinitidine metabolites ; Deproteinisation on precolumn ; Column switching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary New quinidine metabolites, including 10,11-dihydrodiol quinidine N-oxide, 10,11-dihydrodiol quinidine and their glucuronides, were found in human urine. A quinidine monitoring HPLC method including these metabolites, is proposed by the direct injection of body fluid samples onto the precolumn for deproteinization followed by reverse phase separation in the analytical column with a column switching technique. The recovery of spiked quinidine and its metabolites in plasma was quantitative (98–102%) with good reproducibility (C.V.: 1.6–4.0%). Several clinical samples such as whole blood and urine were analyzed by the present method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262689
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    HPLC analysis of human epidermal growth factor using immunoaffinity precolumn. I. Optimization of immunoaffinity column (1989)
    Springer
    Chromatographia 27 (1989), S. 569-573 
    Details
    ISSN: 1612-1112
    Keywords: Immunoaffinity chromatography ; Human epidermal growth factor (hEGF) ; Immobilization of antibody ; Pore size of diol silica ; Optimum coupling conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The preparation of support-coupled antibodies for high-performance immunoaffinity chromatography has been demonstrated. Diol silica had the least non-specific adsorption of all supports investigated. The optimum activation conditions for diol silica with 1,1′-carbonyl-diimidazole and the optimum coupling conditions for anti-human epidermal growth factor antibody as a function of salt concentration, pH and ligand concentration were selected. The pore size of the diol silica was an important factor in achieving good results. Diol silica with 500Å pore size had high loading capacity against the antigen. The use of F(ab′)2 or Fab as a ligand showed almost the same chromatographic characteristics as IgG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258980
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    On-line deproteinization of serum sample for HPLC analysis of hydrophilic compounds and its application to gentamicin (1986)
    Springer
    Chromatographia 21 (1986), S. 519-522 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; On-line deproteinization ; Butyl Toyopearl 650-M ; Gentamicin in serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The binding of serum proteins with Butyl Toyopearl (BT) 650-M has been investigated and applied to on-line deproteinization for the HPLC determination of gentamicin components, c1, c1a, c2, in serum. It was found that in 0.4% perchloric acid medium about 36mg of BSA was adsorbed on 1ml of wet gel. Under this condition hydrophilic components such as gentamicin passed through the pre-column packed with BT 650-M, while serum proteins and hydrophobic components were trapped in the pre-column. The ion pair between gentamicin components and pantanesulfonate anion was effectively trapped in a reversed-phase analytical column. It was then eluted and fluorometrically determined by post-column derivatization with o-phthalaldehyde. The recovery was quantitative with good reproducibility at therapeutic concentrations in sera. Several clinical samples were analyzed by the method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310539
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    HPLC analysis of human epidermal growth factor using immunoaffinity precolumn. II. Determination of hEGFs in biological fluids (1989)
    Springer
    Chromatographia 27 (1989), S. 574-580 
    Details
    ISSN: 1612-1112
    Keywords: Immunoaffinity chromatography ; Human epidermal growth factor (hEGF) ; Degradation products ; Immunoaffinity precolumn ; HPLC column-switching system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid-chromatographic, column-switching system for automated sample pre-treatment and determination of human epidermal growth factor and its degradation products (hEGFs) is described. The system consists of an immunoaffinity precolumn (4.0×10mm) packed with diol silica immobilized with antibody against hEGF and an analytical ODS column (4.6×250mm). Samples such as cultured media ofE. coli, human urine, milk, seminal fluid and saliva can be directly injected on the immunoaffinity precolumn and the analytes of interest are trapped by the immobilized anti-hEGF antibody. After washing this precolumn with aqueous solvents, the analytes are desorbed with an aqueous solution of low pH and transferred to the analytical column to allow their separation in reversed phase mode. The recoveries of hEGFs spiked in these biological fluids were over 98%. The detection limit was 1 ng for a 1ml sample injection. This method was applied for the determination of hEGF levels in cultured media ofE. coli and biological fluids. Degradation of hEGF in human serum and urine was also examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258981
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    Paper (German National Licenses)
    Fulltext
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