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  • Electronic Resource  (4)
  • Confocal laser scanning microscopy  (2)
  • Ehrlich ascites tumor  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Induction of cell arrest at G1/S and in G2 after treatment of Ehrlich ascites tumor cells with metallocene dichlorides and cis-platinum in vitro (1983)
    Springer
    Journal of cancer research and clinical oncology 106 (1983), S. 44-52 
    Details
    ISSN: 1432-1335
    Keywords: Metallocene dichlorides ; cis-Diammine-dichloroplatinum(II) ; Ehrlich ascites tumor ; Cytokinetics in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of in vitro application of the cytostatic agents titanocene dichloride (TDC), vanadocene dichloride (VDC), and cis-diamminedichloroplatinum(II) (DDP) on the cytokinetic behavior of EAT cells was investigated. All three substances induced similar cytokinetic phenomena that are characterized on the one hand, by an accumulation of cells in the late S phase and in the G2 phase during exposure periods of 8 to 20 h. On the other hand, some cells were apparently arrested at the end of the G1 phase and, after removal of the cytostatic agents, moved through the following S, G2, and M phases as synchronized cell populations. In view of this unusual cytokinetic pattern of multiple effects, it is argued that the striking similarities in cytokinetic behavior after treatment with the three different cytostatic substances indicate analogies in their molecular mechanisms of action. The different alteration patterns observed after in vivo application of the drugs are obviously due to additional processes occurring only in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399896
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  • 2
    Electronic Resource
    Electronic Resource
    Histotomographische Untersuchungen zur Frage direkt radiogener Schmelzveränderungen (1998)
    Springer
    Mund-, Kiefer- und Gesichtschirurgie 2 (1998), S. 85-90 
    Details
    ISSN: 1434-3940
    Keywords: Schlüsselwörter Strahlenkaries ; In-vitro-Demineralisation ; Konfokale Laser-Scanning-Mikroskopie ; Key words Radiation caries ; In vitro demineralisation ; Confocal laser scanning microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Objective: The volume of literature on radiation-induced caries is still inconsistent as regards the state of the art. Our histotomographic studies have shown direct radiogenic effects on the dentoenamel junction (atrophy of the odontoblast processes). Whether there are direct effects of radiation on dental enamel is not yet known. Material and Method: Sound teeth (group 1, n = 10) were compared either with enamel specimens after high dose in vitro radiation (500– 2500 Gy; group 2; n = 10) and after experimental enoral in situ irradiation (60 Gy; group 3; n = 20), or with teeth from patients after cancer radiotherapy (36 Gy; group 4; n = 20) and with teeth which had obviously decayed due to cancer radiotherapy (60 Gy; group 5; n = 20). Vestibular enamel surfaces of the teeth were exposed to identical in vitro demineralizations (lactic acid gel; pH = 5.0). Each of the samples was prospectively studied histologically by confocal laser scanning microscopy (CLSM) after 90, 180 and 270 min of acid interaction. Data were interpreted micromorphometrically (width of the demineralized area) and micromorphologically. Results: The width of the demineralized area varies between the two in vivo irradiated groups of specimens (group 4: P 〈 0.01; group 5: P 〈 0.001) and the high dose in vitro irradiated teeth (P 〈 0.01) and is significantly different from the sound controls. The histological image of the demineralization in subsurface areas in the teeth of radiotherapy patients is characterized by a total loss of the prismatic structure (homogeneously, amorphous substance). Conclusions: Direct radiogenic effects at the dentoenamel junction have been described earlier. The additional information offered by this study is that there are significant micromorphometric differences in the demineralizing behaviour of irradiated enamel. Obviously, enamel is less resistant to acid attack after irradiation.
    Notes: Fragestellung: Das umfangreiche Schrifttum zur Strahlenkaries zeigt den kontroversen Forschungsstand. Eigene Untersuchungen haben direkt radiogene Veränderungen an der Schmelz-Dentin-Grenze histotomographisch nachweisen können (Atrophie der Odontoblastenfortsätze). Offen ist die Frage nach direkten Strahlenfolgen im Schmelz. Material und Methode: Gesunde Zähne (Gr. 1, n = 10) wurden mit Proben nach hochdosierter In-vitro- (500–2500 Gy, Gr. 2, n = 20) und nach experimentell-enoraler In-situ-Bestrahlung (60 Gy, Gr. 3, n = 20) sowie mit Zähnen von Patienten nach präoperativer Strahlentherapie (36 Gy, Gr. 4, n = 20) und strahlenkariösen Zähnen nach tumortherapeutischer Strahlendosis (60 Gy, Gr. 5, n = 20) verglichen. Im direkten Parallelgruppenvergleich erfolgte eine standardisierte In-vitro-Demineralisierung (Milchsäuregel pH 5,0) an den vestibulären Schmelzoberflächen. Jede Probe wurde prospektiv histologisch durch konfokale Laser-Scanning-Mikroskopie (CLSM) nach 90, 180 und 270 min Entmineralisierung untersucht. Die Auswertung erfolgte mikromorphometrisch (Breite des Demineralisationssaums) und mikromorphologisch. Ergebnisse: Die Breite des Demineralisationssaums unterschied sich sowohl bei beiden in vivo bestrahlten Probengruppen (Gr. 4: p 〈 0,01; Gr. 5: p 〈 0,001) als auch bei den hochdosiert in vitro bestrahlten Zähnen (p 〈 0,01) signifikant von den Befunden der gesunden Kontrollen. Das histologische Bild der Demineralisation an den Zahnoberflächen von Radiotherapiepatienten zeigte dabei einen völligen Verlust der Prismenstruktur (homogen amorphe Substanz). Schlußfolgerungen: Direkte Strahlenfolgen an der Schmelz-Dentin-Grenze wurden in früheren Untersuchungen dargestellt. Zusätzlich konnten jetzt signifikante mikromorphometrische Unterschiede im Demineralisierungsverhalten des bestrahlten Schmelzes gezeigt werden. Offensichtlich ist der Schmelz nach der Bestrahlung für einen Säureangriff weniger resistent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100060050035
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  • 3
    Electronic Resource
    Electronic Resource
    Cytokinetic behavior of Ehrlich ascites tumor after in vivo treatment with cis-diamminedichloroplatinum(II) and metallocene dichlorides (1981)
    Springer
    Journal of cancer research and clinical oncology 102 (1981), S. 21-30 
    Details
    ISSN: 1432-1335
    Keywords: cis-Diamminedichloroplatinum(II) ; Titanocene dichloride ; Vanadocene dichloride ; Ehrlich ascites tumor ; Pulse-cytophotometry ; cis-Diammindichloroplatin(II) ; Titanocen-dichlorid ; Vanadocen-dichlorid ; Ehrlich-Aszites-Tumor ; Impulscytophotometrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Gegenstand der Untersuchungen ist der Einfluß einer in-vivo-Behandlung mit optimal tumorhemmenden Dosen von cis-Diammindichloroplatin(II) (DDP), Titanocen-dichlorid (TDC) und Vanadocen-dichlorid (VDC) auf das impulscytophotometrisch bestimmte DNA-Verteilungsmuster und die Mitoseaktivität von EAT-Zellen. Während DDP eine sofortige und langandauernde Unterdrückung der Zellteilungen, jedoch keine Veränderung des zellkinetischen Verhaltens von EAT-Zellen bewirkt, verursacht TDC die Ausbildung eines deutlichen,: 10–12 h nach Behandlung maximalen G2-Blocks. Im Falle von DDP und TDC werden die Tumorzellen einige Tage nach Behandlung durch Zellen des wirtstiereigenen Abwehrsystems beseitig. VDC hingegen ruft die Entstehung teilsynchroner Wellen nach einer vorübergehenden Mitosehemmung und einer reversiblen Zellakkumulation in der späten S-und der G2-Phase hervor. Darüber hinaus werden 5–16% der Zellen nach VDC-Behandlung polyploid.
    Notes: Summary The influence of an in vivo treatment with optimally tumor-inhibiting doses of cis-diamminedichloroplatinum(II) (DDP), titanocene dichloride (TDC), and vanadocene dichloride (VDC) on the DNA distribution pattern, determined by pulse-cytophotometry, and on the, mitotic activity of EAT cells is investigated. Whereas DDP causes an immediate and, long-lasting decrease of mitoses but no disturbances of the EAT cell kinetics, a marked G2 block with a maximum at 10–12 h a. t. and an irreversible and extensive mitotic depression is evoked by TDC. In the case of DDP and TDC, the tumor cells are removed several days a. t. by cells belonging to the defensive system of the host animals. In contrast, VDC induces partially synchronized waves after a transient suppression of mitoses and reversible cell accumulation in the late S and in the G2 phases. Additionally, 5–16% of the cells become polyploid after VDC treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410531
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  • 4
    Electronic Resource
    Electronic Resource
    Histotomography of the odontoblast processes at the dentine–enamel junction of permanent healthy human teeth in the confocal laser scanning microscope (1998)
    Springer
    Clinical oral investigations 2 (1998), S. 21-25 
    Details
    ISSN: 1436-3771
    Keywords: Key words Dentine ; enamel junction ; Odontoblast processes ; Dentinal tubules ; Confocal laser scanning microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The translucency of teeth allows the non-destructive subsurface visualisation of their microstructure by confocal laser scanning microscopy (CLSM) at a level of about 150 μm below the surface. The dentine–enamel junction (DEJ) is accessible only directly adjacent to the cervix of the tooth. Therefore teeth have to be sectioned for studying marginal areas of the dental hard tissue. The potential of the technique for (pseudo) three-dimensional visualisation allows the study of an array of individual confocal images, the interpretation of which is similar to that of macroscopic tomographs (CT-scan, MRI). Additionally, the extended focus mode yields the overlay of individual confocal images in the form of a two-dimensional projection. This mode of operation proved to be particularly suited for the visualisation of odontoblast processes in their whole extension. The three-dimensional junction between enamel and dentine, the branches of the odontoblast processes and their interactions with the DEJ is demonstrable by CLSM without staining or other procedures of sample preparation. The direct microscopic comparison between samples, either fresh or kept in a humid chamber, and Technovit-embedded sample blocks gives evidence that the risk of artefacts by sample storage or by the embedding procedure is minimal. The tomographs limited to subsurface areas of the tissue also exclude mechanical surface artefacts due to grinding or cutting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007840050038
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