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  • Electronic Resource  (8)
  • Pinus  (3)
  • Polymer and Materials Science  (3)
  • Dominant markers  (2)
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  • Electronic Resource  (8)
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  • 1
    Electronic Resource
    Electronic Resource
    Contrasting patterns of genetic diversity in two tropical pines: Pinus kesiya (Royle ex Gordon) and P. merkusii (Jungh et De Vriese) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 436-441 
    Details
    ISSN: 1432-2242
    Keywords: Pinus ; Allozymes ; Chloroplast DNA ; Genetic variation ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied allozyme and chloroplast (cp) DNA variation in natural populations of Pinus kesiya and P. merkusii from Thailand and Vietnam. The results showed striking differences between the two species in the amount and distribution of allozyme variation. P. kesiya harboured considerable allozyme variation and showed weak interpopulational differentiation. In contrast, P. merkmii had very low intrapopulational variability but a high level of interpopulational differentiation. The average Nei's genetic distance separating the two species was exceptionally high (0.701) taking into account their close taxonomic placement in the same subsection Sylvestres. The constructed phylogenetic trees revealed very early divergence of P. kesiya and P. merkusii. The present analysis of cpDNA variation also confirmed the dissimilar character of these two species and was compatible with other evidence indicating the outstanding position of P. merkusii as compared to other Asian members of the subsection Sylvestres. Analysis of cpDNA variation in sympatric populations of P. kesiya and P. merkusii revealed that they are pure representatives of the species in question. This result indicates that despite an overlapping distribution P. kesiya and P. merkusii do not hybridise in nature. We suggest that the distinctive character of P. merkusii is a result of an early separation from other Eurasian pines. Despite spatial proximity, P. kesiya and P. merkusii are kept apart by strong reproductive barriers. The low genetic variability of P. merkusii may be explained by previous bottlenecks, reduced gene flow among populations, and an inbreeding due to small population size and asynchronous flowering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223690
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  • 2
    Electronic Resource
    Electronic Resource
    Evolutionary analysis of Pinus densata (Masters), a putative Tertiary hybrid. (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 641-647 
    Details
    ISSN: 1432-2242
    Keywords: Pinus ; Species hybridization ; Chloroplast DNA ; Molecular markers ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment analysis and heterologous hybridization of chloroplast (cp) DNA was used to develop species-specific markers for P. tabulaeformis, P. yunnanensis and P. massoniana. Fragment patterns created by the BclI and DraI restriction enzymes and hybridization patterns to the psbC and psbD probes were distinctive among the three species. No intraspecific variation was detected with respect to any of the cpDNA markers developed in this study. The cpDNA markers obtained were subsequently used to examine the parentage of P. densata, a putative Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The analysis demonstrated for the first time that P. densata populations accommodate chloroplast genomes of P. tabulaeformis and P. yunnanensis, which strongly supports earlier suggestions of the hybrid origin of this species. It appears that P. densata represents a stabilized natural hybrid that has become adapted to high mountain environments where neither of the parental species can normally grow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224224
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  • 3
    Electronic Resource
    Electronic Resource
    Evolutionary analysis of Pinus densata Masters, a putative Tertiary hybrid (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 635-640 
    Details
    ISSN: 1432-2242
    Keywords: Pinus ; Species hybridization ; Allozymes ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Allozyme differentiation at 13 loci was studied in populations of Pinus tabulaeformis, P. densata, and P. yunnanensis from China. It was previously suggested that P. densata represents a Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The observed levels of allozyme variation within and among the investigated species were comparable to those of other conifers. P. tabulaeformis differed markedly from P. yunnanensis with respect to allozyme frequencies, while P. densata was intermediate between the two putative parents. There was evidence of homozygote excess in embryos from all investigated species, as compared to Hardy-Weinberg expectations. The observed allozyme composition of P. densata conformed to earlier morphological and molecular evidence indicating hybrid origin of this taxon. It was proposed that fusion of gene pools from P. tabulaeformis and P. yunnanensis has led to adaptive evolution of a new species, P. densata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224223
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  • 4
    Electronic Resource
    Electronic Resource
    TransgenicArabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The map positions of a set of eight T-DNA insertions in theArabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar),β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). Theneo, hpt andbar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing thehpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172528
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  • 5
    Electronic Resource
    Electronic Resource
    Transgenic Arabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Keywords: Key words Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar), β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172528
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    On the gel effect in the presence of a chain transfer agent in methyl methacrylate polymerization and its copolymerization with various acrylates (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 49 (1993), S. 2179-2188 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The kinetics of methyl methacrylate (MMA) polymerization, and of its copolymerization with various acrylates, at high conversions in the presence of a chain transfer agent, are investigated with a dilatometer over the entire course of reaction. The displacement to higher conversions of the onset of the gel effect in the MMA homopolymerization, in the presence of a chain transfer agent, was determined. Similar information is also provided for the MMA-acrylate copolymerization systems. An increase in polymerization temperature slightly delays the onset of the gel effect in the MMA-acrylate copolymerization, but considerably increases the final conversion. The final conversion in copolymerization for a constant concentration of the chain transfer agent is independent of the initiator concentration, but is a function of the polymerization temperature. The reaction time for reaching the limiting conversion in copolymerization is increased with an increasing amount of the second monomer, as well as with an increasing number of carbon atoms in the acrylate used as the second monomer. © 1993 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1993.070491214
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  • 7
    Electronic Resource
    Electronic Resource
    XPS analysis of hydroxide ion surface reactions on reactions on CeF3 and LaF3 fluoride ion-selective electrodes (1997)
    Weinheim : Wiley-Blackwell
    Electroanalysis 9 (1997), S. 917-921 
    Details
    ISSN: 1040-0397
    Keywords: XPS analysis ; Fluoride ; Ion selective electrode ; Surface analysis ; Hydroxide interference ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Lanthanum fluoride and cerium fluoride single crystals, used as the sensing membranes of the fluoride ion-selective electrode (F-ISE), were investigated for their hydroxide interference and surface reactions with OH- in high pH conditions. While these membranes show fast response and excellent Nernstian behavior over a wide concertration range in buffered F- solutions, they deviate from the theoretical slope at high pH. CeF3, in particular, exhibits a much larger deviation from the Nernstian slope and a substantially slower response to a F- activity change compared to LaF3. This larger deviation is due to more extensive and faster formation of hydroxo-complexes, with the release of a greater amount of the fluoride ion into the hydrated gel layer. The slower response of the CeF3 membrane after contact with OH- is due to the formation of CeIV oxide on the surface, which acts as a blocking layer to the exchange of F-.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140091210
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  • 8
    Electronic Resource
    Electronic Resource
    Porous-coated titanium implant impregnated with a biodegradable protein delivery system (1997)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 36 (1997), S. 516-521 
    Details
    ISSN: 0021-9304
    Keywords: porous-coated ; titanium ; PLA-PGA ; protein release ; degradation ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Tissue ingrowth into porous-coated orthopedic and dental implants is commonly used as a means to achieve long-term fixation of these prostheses. However, the degree of tissue ingrowth is often inadequate and inconsistent. If the pores of these implants are impregnated with a controlled drug release system delivering relevant growth factors, then it might be possible to stimulate more tissue ingrowth. The present study introduces such a system based on biodegradable polymers and investigates its protein release profile and polymer degradation characteristics. Porous coated titanium implants were impregnated with a mixture of a 50%-50% polylactic acid-polyglycolic acid copolymer and a model protein, soybean trypsin inhibitor. Control implants contained only the polymer and no protein. The implants were subjected to hydrolytic degradation in phosphate buffered saline at 37°C for periods of 3, 6, and 11 weeks. The protein release and the mass and molecular weight of the polymer were monitored. The results indicate that the protein is released in three distinct phases and the polymer loses almost all its mass and molecular weight by 11 weeks. There was a significant difference in the polymer degradation characteristics between the control and test implants, which might be the result of some complex polymer-protein interactions. © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 36, 516-521, 1997.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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