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  • Electronic Resource  (2)
  • Food  (1)
  • Industrial Chemistry  (1)
  • 1
    ISSN: 1438-2385
    Keywords: Key words Polymerase chain reaction ; Campylobacter ; Internal standard ; Food ; False negatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A heterologous internal standard, termed "mimic", was developed for the polymerase chain reaction (PCR)-based detection of Campylobacter jejuni and Campylobacter coli in food. Mimic was designed to contain a heterologous DNA fragment of plasmid pUC18, flanked by a primer binding site, identical to the bacterial target DNA. Application of mimic in the PCR permitted its co-amplification together with the bacterial DNA with similar efficiency. As the length of the amplified products differed, they were easily detectable by agarose gel electrophoresis. The presence or absence of the mimic PCR product was indicative of the efficacy of the PCR. The use of approximately 60 mimic molecules per reaction was optimal for determining the reliability of the diagnostic PCR assays without decreasing the detection limit. This system for the detection of the two species of Campylobacter was successfully applied in routine food surveillance.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 39 (1967), S. 291-296 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In der Nähe des kritischen Zustandes bildet sich infolge der hohen Kompressibilität in einem ruhenden fluiden Medium eine mit der Höhe veränderliche Dichte aus. Diese Dichteschichtung wurde an CO2, N2O und CF3Cl sowie an Gemischen aus diesen Stoffen über die örtliche Brechzahl bestimmt. Die Versuchsapparatur wurde besonders sorgfältig auf konstanter Temperatur gehalten, so daß einwandfreie Gleichgewichtszustände erzielt wurden. Aus den Ergebnissen lassen sich die Grenzkurve, die Dampfdruckkurve und das Druck-Dichte-Diagramm in der Nähe des kritischen Zustandes mit größerer Genauigkeit bestimmen, als es durch die bisher übliche Messung von Mittelwerten der Dichte möglich war.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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