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  • Digitale Medien  (4)
  • Wheat  (2)
  • Leaf rust  (1)
  • Mutation  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Control of tissue culture response in wheat (Triticum aestivum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 783-787 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Tissue culture ; Callus ; Wheat ; Ditelosomics ; Nullisomic-tetrasomics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond to tissue culture has been shown to involve the group 2 chromosomes. The available group 2 ditelosomic and nullisomic-tetrasomic lines of ‘Chinese Spring’ wheat were used to determine the chromosome arm location and chromosome dosage effect associated with the expression of tissue culture response (TCR). Significant differences were found between the aneuploid lines and the euploid control for the expression of both regenerable callus formation and callus growth rate. A model is proposed suggesting that a major TCR gene is located on 2DL and that 2AL and 2BS possess minor TCR genes. Furthermore, a major regulatory gene controlling the expression of TCR genes may be located on chromosome 2BL.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00266658
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  • 2
    Digitale Medien
    Digitale Medien
    Heritable somaclonal variation in gliadin proteins of wheat plants derived from immature embryo callus culture (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 784-790 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Tissue culture ; Electrophoresis ; Storage proteins ; Mutation ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fertile r0 plants of the winter wheat line ND7532 (Triticum aestivum L.) were regenerated from callus tissue after 60–190 days in culture. Seeds produced from these self-pollinated plants were planted in the field. Of the 5586 R1 plants, 32 differed for one or more agronomic traits from plants not passed through tissue culture process. Gliadin electrophoregrams were prepared from bulk samples of R2 seed from these 32 plants. Four of the 32 produced gliadin patterns different from controls, so 12 seeds of each of these four lines were examined individually. Three of the four mutant lines were fixed for the presence of a mutant protein of 50 relative mobility units (RMU) and the corresponding loss of a parental protein of 26 RMU. The remaining line segregated for the presence/absence of band 50 and the corresponding loss/retention of band 26. The mutant protein of 50 RMU was never seen in control plants. This indicated that either band 50 was coded for by a mutant gene allelic to the gene that coded for band 26 or that bands 26 and 50 were coded for by two different structural alleles under the control of a common regulatory locus. Each of the 12 seeds from the four mutant lines contained a prominent protein band at 30 (RMU), which was only observed as a faint band in one control seed. The types of variation in gliadin patterns observed in somaclones of ND7532 were similar to those reported for the line ‘Yaqui 50E’, except that, gliadin changes occurred less frequently in ND7532.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00276418
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  • 3
    Digitale Medien
    Digitale Medien
    Use of winter wheat x Triticum tauschii backcross populations for germplasm evaluation (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 571-577 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Triticum tauschii ; Aegilops squarrosa Wheat ; Yield ; Protein ; Hardness ; Leaf rust
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The wild diploid goatgrass, Triticum tauschii (Coss.) Schmal., is an important source of genes for resistance to both diseases and insects in common wheat (Triticum aestivum L.) We have evaluated grain yield, kernel weight, protein concentration, and kernel hardness of 641 BC2 F1-derived families from direct crosses involving four T. aestivum cultivars and 13 T. tauschii accessions over 2 years and at two Kansas, USA, locations. On average, T. tauschii germplasm depressed grain yield and increased protein concentration, whereas kernel weight was affected either positively or negatively, depending on the T. tauschii parent. Three T. tauschii parents produced a large proportion of families with very soft endosperm. Some variation among progeny of different T. tauschii parents resulted from the segregation of genes for resistance to leaf rust (caused by Puccinia recondita Rob. ex Desm.). This study confirmed that random BC2-derived families can be used to evaluate the effects of T. tauschii genes in the field. This methodology, although laborious, can provide useful information which is not obtainable by the screening of T. tauschii accessions themselves.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222005
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  • 4
    Digitale Medien
    Digitale Medien
    Development of PCR markers linked to resistance to wheat streak mosaic virus in wheat (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 463-467 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Wheat streak mosaic virus ; Markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (Acer tulipae), is an important disease of wheat (Triticum aestivum L.) in the North American Great Plains. Resistant varieties have not been developed for two primary reasons. First, useful sources of resistance have not been available, and second, field screening for virus resistance is laborious and beyond the scope of most breeding programs. The first problem may have been overcome by the development of resistance to both the mite and the virus by the introgression of resistance genes from wild relatives of wheat. To help address the second problem, we have developed polymerase chain reaction (PCR) markers linked to the WSMV resistance gene Wsm1. Wsm1 is contained on a translocated segment from Agropyron intermedium. One sequence-tagged-site (STS) primer set (WG232) and one RAPD marker were found to be linked to the translocation containing Wsm1. The diagnostic RAPD band was cloned and sequenced to allow the design of specific PCR primers. The PCR primers should be useful for transferring Wsm1 into locally adapted cultivars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223191
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