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  • Digitale Medien  (8)
  • Life and Medical Sciences  (2)
  • N availability index  (2)
  • electrophoresis  (2)
  • non-Hodgkin's lymphoma  (2)
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  • Digitale Medien  (8)
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  • 1
    ISSN: 1569-8041
    Schlagwort(e): chemotherapy ; dose intensification ; long-term survival ; MACOP-B ; non-Hodgkin's lymphoma ; randomized trial
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background: The initial publication of the results of the Australianand New Zealand Lymphoma Group (ANZLG) randomized controlled trial comparingMACOP-B and CHOP in patients with intermediate-grade non-Hodgkin's lymphoma(NHL) showed equivalent complete response rates, time to treatment failure,and survival. Here we report the long-term follow-up of the 236 patientsentered on that study to determine if there were any long-term advantages ordisadvantages associated with MACOP-B. Patients and methods: Two hundred thirty-six eligible patients wererandomized between October 1986 and June 1991. The median duration offollow-up has been extended from 3.2 years in our previous publication to 6.5years. Results: As previously reported, the complete response (CR) rate forMACOP-B and CHOP chemotherapy was 51% and 59%, respectively. Theestimated failure-free survival rate for MACOP-B and CHOP patients was42% and 30%, respectively, at 5 years (P = 0.045) and37% and 25%, respectively, at 8 years (P = 0.057). Theestimated overall survival rate at 5 years was 54% for MACOP-B and41% for CHOP patients (P = 0.035) and at 8 years was 45%and 36%, respectively (P = 0.16). Conclusion: With this extended follow-up, we have shown a long-termsurvival advantage for MACOP-B chemotherapy over standard CHOP inpatients with intermediate-grade non-Hodgkin's lymphoma.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1569-8041
    Schlagwort(e): cyclophosphamide ; dose-escalation ; epirubicin ; filgrastim ; G-CSF ; non-Hodgkin's lymphoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background: To define a maximum tolerated dose (MTD) for the combination of epirubicin and cyclophosphamide with filgrastim (r-met-HuG-CSF) in patients with advanced solid tumors and non-Hodgkin's lymphoma (NHL). Patients and methods: Thirty-five patients with advanced solid tumors were enrolled in stages I and II. Twenty-one patients were treated in stage I in sequential cohorts of at least three patients at increasing dosage levels of cyclophosphamide and epirubicin, for up to six cycles every 21 days. At the completion of stage I, a MTD for epirubicin was established. Fourteen patients were treated in stage II, in cohorts of three or more. The epirubicin dose remained constant at the MTD dosage from stage I. Cyclophosphamide was further dose-escalated to establish its MTD. Twenty-one patients with previously untreated non-Hodgkin's lymphoma were treated in stage III with the MTD established in the prior stages. Results: The MTD in stage I was epirubicin 150 mg/m2 and cyclophosphamide 1500 mg/m2 with cumulative neutropenia as the dose-limiting toxicity (DLT). Cumulative thrombocytopenia prevented further dose-escalation of cyclophosphamide in stage II. The stage III regimen consisted of six, 21-day cycles of epirubicin 150 mg/m2, cyclophosphamide 1500 mg/m2, vincristine 2 mg, and prednisolone 100 mg for five days with filgrastim support. Nineteen of twenty-one patients (90%) completed six cycles of treatment, eight (38%) without dose reduction. Common toxicity criteria (CTC) grade 4 neutropenia (neutrophil nadir 〈0.5 × 109/l) was documented in 85 of 118 cycles (72%). Neutropenic fever was documented in 17 of 21 patients (81%) on at least one occasion. Severe thrombocytopenia (〈25 × 109/l) was seen in fourteen of 118 cycles (12%) and increased with cycle number. There was no significant non-hematological toxicity. Conclusion: Significant dose-escalation of epirubicin and cyclophosphamide was possible with filgrastim support. The MTD achieved was approximately double that of standard-dose therapy. This study forms the basis of an ongoing randomized study evaluating dose-intensification in intermediate grade NHL.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 21 (1983), S. 177-189 
    ISSN: 1573-4927
    Schlagwort(e): β-d-galactosidase ; β-d-glucosidase ; electrophoresis ; genetics ; rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Three different types of β-d-galactosidase (EC 3.2.1.23) could be distinguished in rabbit tissues using electrophoretic procedures. (1) Acid β-d-galactosidase with a low mobility and maximal activity at pH 3–5 was found in the particulate fraction of various tissue homogenates. This enzyme hydrolyzed 4-methylumbelliferyl-d-galactoside, but no activity against other glycoside substrates could be demonstrated. The enzyme was inhibited by galactono-(1 → 4)-lactone. (2) Lactose-hydrolyzing β-d-galactosidase with an intermediate mobility was found only in juvenile small intestine. Most of the activity was found in the particulate fraction of the cell. The enzyme hydrolyzed several other synthetic glycoside substrates besides lactose. It was most active at pH 5–6 and strongly inhibited by glucono-(1 → 5)-lactone but not much affected by galactono-(1 → 4)-lactone. (3) Neutral β-d-galactosidase with a fast mobility and maximal activity at pH 6–8 was found in the soluble fraction of homogenates from liver, kidney, and small intestine. This enzyme also showed a broad substrate specificity; it possessed activity against aryl-β-d-glucoside, -fucoside, and -galactoside substrates but not against lactose. The enzyme was strongly inhibited by glucono-(1 → 5)-lactone and (less) by galactone-(1 → 4)-lactone. Neutral β-d-galactosidase and neutral β-d-glucosidase (EC 3.2.1.21) are probably identical enzymes in the rabbit. Individual variation, in both electrophoretic mobility and activity, was found for neutral β-d-galactosidase. Genetic analysis of the electrophoretic variants revealed that two alleles at an autosomal locus are responsible for this variation.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 21 (1983), S. 177-189 
    ISSN: 1573-4927
    Schlagwort(e): β-d-galactosidase ; β-d-glucosidase ; electrophoresis ; genetics ; rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Three different types of β-d-galactosidase (EC 3.2.1.23) could be distinguished in rabbit tissues using electrophoretic procedures. (1) Acid β-d-galactosidase with a low mobility and maximal activity atpH 3–5 was found in the particulate fraction of various tissue homogenates. This enzyme hydrolyzed 4-methylumbelliferyl-d-galactoside, but no activity against other glycoside substrates could be demonstrated. The enzyme was inhibited by galactono-(1 → 4)-lactone. (2) Lactose-hydrolyzing β-d-galactosidase with an intermediate mobility was found only in juvenile small intestine. Most of the activity was found in the particulate fraction of the cell. The enzyme hydrolyzed several other synthetic glycoside substrates besides lactose. It was most active atpH 5–6 and strongly inhibited by glucono-(1 → 5)-lactone but not much affected by galactono-(1 → 4)-lactone. (3) Neutral β-d-galactosidase with a fast mobility and maximal activity atpH 6–8 was found in the soluble fraction of homogenates from liver, kidney, and small intestine. This enzyme also showed a broad substrate specificity; it possessed activity against aryl-β-d-glucoside, -fucoside, and -galactoside substrates but not against lactose. The enzyme was strongly inhibited by glucono-(1 → 5)-lactone and (less) by galactone-(1 → 4)-lactone. Neutral β-d-galactosidase and neutral β-d-glucosidase (EC 3.2.1.21) are probably identical enzymes in the rabbit. Individual variation, in both electrophoretic mobility and activity, was found for neutral β-d-galactosidase. Genetic analysis of the electrophoretic variants revealed that two alleles at an autosomal locus are responsible for this variation.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 64 (1982), S. 331-341 
    ISSN: 1573-5036
    Schlagwort(e): Humic substances ; Molecular weight distribution ; N availability index ; Ninhydrindetectable N ; Relative N uptake ; Soil proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two soil extracts used for chemical indexes for N availability, 0.01M NaHCO3 and boiling 0.01M CaCl2, were analyzed in effort to learn more about the nature of the extracted organic matter (O.M.). The two extracts appeared to remove different fractions of the soil O.M. A study of five soils showed that the C/N value of the NaHCO3 extract (following decarbonation) was significantly higher than that of the total soil O.M.; while the C/N value in the boiling CaCl2 extract was not significantly different from that in the soil O.M. There was also significant variation in C/N values among soils for the boiling CaCl2 extract. The extracts of three soils were analyzed for apparent molecular weight distribution using gel filtration and the results compared to those for base-extracted humic substances. Almost all the molecules in the extracts had apparent molecular weights less than 21,000 daltons while 21 to 47% of the humic substances from the same soils (extracted with 0.5M NaOH) had molecular weights greater than 21,000 daltons. In the boiling CaCl2 extract, 78 to 87% of the humic substances had apparent molecular weights less than 1,000 daltons, whereas with the NaHCO3 extract, 42 to 83% of the humic substances were in the 1,000 to 21,000 dalton range. Forty-three to 92% of the N extracted by the NaHCO3 was in protein form, and 8 to 30% was ninhydrin-detectable. In the boiling CaCl2 extract 25 to 30% of the extracted N was ninhydrin-detectable. For the same 10 soils, ninhydrin-detectable N values of the boiling CaCl2 extract appeared closely related to greenhouse and field relative N uptake, while the ninhydrin-detectable N values of the NaHCO3 extract appeared unrelated to both. The protein N and protein in plus ninhydrin-detectable N values of the NaHCO3 extract were closely related to greenhouse relative N uptake only. The results of this study indicated that specific fractions of the soil O.M. were being extracted by the two solutions and that significant differences existed in the chemical nature of the two extracts.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 66 (1982), S. 81-89 
    ISSN: 1573-5036
    Schlagwort(e): Extractable organic matter ; N availability index ; Organic matter ; Organic N ; Soil storage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The effect was studied of storage temperature on the index of available soil N wich uses U.V. absorbance of a 0.01M NaHCO3 extract as an indicator. The U.V. absorbance was found to increase at a non-linear rate for four soils stored at temperatures of 50, 75, and 150°C. The change in extract absorbance due to extended soil storage at each of these temperatures was positively correlated to the percent organic matter, percent N, C/N value and concentration of humic substances in soils, but not to the extract absorbance prior to soil storage. These findings were not consistent with room temperature storage data which showed a linear increase in extract absorbance with soil storage time. The change in absorbance for the room temperature case was not related to any of the soil parameters mentioned above. Analysis of a soil stored at 105°C showed an increase in ninhydrin-detectable N, protein N and Kjeldahl N of the NaHCO3 extract, while the apparent molecular weight distribution of extracted organic matter (as determined by gel filtration) showed only a slight change. As a comparison to the NaHCO3 extract, a boiling CaCl2 extract of the same soil was also analyzed; and the absorbance at 260 nm was found to increase in a curvilinear fashion with starage time at 75°C but to less of an extent than was noted with the NaHCO3 extract. Nitrogen availability indexes based on the U.V. absorbance of these extracts, particularly those utilizing the NaHCO3 extract, would be significantly affected by soil storage at elevated temperatures.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 128 (1970), S. 137-145 
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Significant sex and strain differences in correlation of bone, body and skeletal weights show significant localized effects of sex and strain and a combination of both. Individually they seem to lack biological meaning. However, when considered in relation to the ponderal correlations, of which they are a part, they fall into place at the distal ends of a graded linear correlation series, both within and between limbs; and similarly between mandible and sacrum, respectively, with each bone of both limbs. The pattern of these gradients reveals a linear decline in correlation of bones with distance (rule of neighborhood, Karl Pearson) in most cases, but the exceptions are such as can be related to the functions in which they cooperate. In this way they demonstrate at least two major unifying genetic influences. One associates sex and strain with locomotion and the other with the incipient upright investigative posture of the Lagomorphs (including the rabbit). The expected effect of sex on the pelvis is apparently not expressed by these ponderal measurements. The manner in which this correlation approach, based on well established genetic growth differences, converges with the phylogenetic and ontogenetic concepts of normal and abnormal growth of bone in limbs and cranio-facial development suggests that a combination of correlation and epigenetic analysis would be helpful in establishing a sound genetic background for the newer biomechanic, functional matrix and architectonic approaches.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 2 (1984), S. 161-168 
    ISSN: 0736-0266
    Schlagwort(e): Rabbit ; Spondylosis ; Calcified nucleus pulposus ; Intervertebral disc degeneration ; Life and Medical Sciences
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The spines of 35 rabbits (32 New Zealand white and 3 AC/J), ranging in age from 3 months to 8½ years, were investigated systematically for spontaneous degenerative changes. Three types of lesion were observed. (1) The nucleus pulposus underwent chondroid metaplasia throughout the length of the vertebral column by the age of 2 years. (2) Hydroxyapatite deposition was found in the nucleus pulposus in 12 of 20 animals examined roentgenographically. The lesion occurred principally in the distal thoracic segments and was first observed in 3-month-old rabbits. (3) Spondylosis occurred in each of four macerated spines from animals 〉24 months old. Portions of the spine spared by disc calcification were affected.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
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