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  • Electronic Resource  (3)
  • Molecular Cell Biology  (1)
  • Short-circuit current  (1)
  • fish cytogenetics  (1)
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  • Electronic Resource  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for a rapid, direct effect on epithelial monolayer integrity and transepithelial transport in response to Salmonella invasion (1996)
    Springer
    Pflügers Archiv 432 (1996), S. 225-233 
    Details
    ISSN: 1432-2013
    Keywords: Key words MDCK epithelium ; Salmonella ; Invasion ; Short-circuit current
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  In cultured monolayers of high-resistance Madin-Darby Canine Kidney (MDCK) cells, infection with Salmonella typhimurium SL1344 resulted in a dose- and time-dependent increase in transepithelial conductance (G t) and short-circuit current (I sc). There was a direct linear relationship between the S. typhimurium-induced increments in I sc and G t suggesting that this early change in epithelial parameters is, in part, the result of a cellular conductance change most probably at the apical membrane. An additional wild-type S. typhimurium strain, SR11, and an invasion-deficient isogenic mutant SB111 carrying a non-polar mutation in invA were used to confirm that the S. typhimurium-induced change in epithelial electrical parameters is directly linked to the invasion process. The S. typhimurium-induced change in epithelial electrical parameters was markedly attenuated in Na+-free choline medium. Addition of piretanide (10–4 M, basal side) failed to affect the increased epithelial conductance and I sc after a 40-min incubation with S. typhimurium. NPPB (5×10–4 M) added to the apical medium reduced the S. typhimurium-stimulated I sc by 28%, but G t was not significantly reduced. It is unlikely that the S. typhimurium-induced I sc is due to Cl–secretion. Staining of S. typhimurium-infected MDCK I monolayers with TRITC-phalloidin revealed marked alterations of F-actin; diffuse intracellular accumulations of F-actin corresponding to the presence of invading bacteria were observed by 15 min. After 60 min, prominent extrusions of the apical membrane corresponding to previously described ”membrane ruffles” were noted. Marked accumulations of perijunctional F-actin in infected cells corresponded to contraction of the perijunctional actin ring at the apical pole. In adjacent cells marked distortion and stretch of the apical surface was evident. The invasion-deficient invA mutant SB111 failed to induce these morphological changes. These data demonstrate that S. typhimurium invasion induces increased transcellular conductance which does not result from stimulation of Cl–secretion but instead appears to be predominantly due to increased Na+ permeability. The increased membrane conductance is coincident with increased transepithelial inulin permeability indicating that the increment in G t has an additional ”paracellular” component. The S. typhimurium-induced alterations in epithelial parameters may be related to ”membrane ruffling” and/or to the accompanying changes in cell shape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050128
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal Characteristics of Ribosomal DNA in the Primitive Semionotiform Fish, Longnose Gar Lepisosteus osseus (1999)
    Springer
    Chromosome research 7 (1999), S. 475-480 
    Details
    ISSN: 1573-6849
    Keywords: chromomycin A3-banding ; FISH ; fish cytogenetics ; GC- rich heterochromatin ; longnose gar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC- rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag- staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in ‘holostean’ and teleostean fishes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009202030456
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The biochemistry of an acetylcholine receptor (1974)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 2 (1974), S. 582-592 
    Details
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The acetylcholine receptor from Torpedo californica electroplax has been studied at three levels of molecular organization: receptor-rich membrane fragments, solubilized and purified receptor, and reconstituted receptor in phospholipid vesicles. The binding of cholinergic ligands to the membrane-bound and the solubilized material is not cooperative, and the number of ligand sites is less than the number of toxin sites. In addition, the purified macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified receptor and Torpedo phospholipids.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400020506
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    Paper (German National Licenses)
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