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Chromosomal Characteristics of Ribosomal DNA in the Primitive Semionotiform Fish, Longnose Gar Lepisosteus osseus (1999)

Ráb, P. ; Rábová, M. ; Reed, K. M. ; [et al.]

Springer

Chromosome research 7 (1999), S. 475-480

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ISSN: 1573-6849

Keywords: chromomycin A3-banding ; FISH ; fish cytogenetics ; GC- rich heterochromatin ; longnose gar

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC- rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag- staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in ‘holostean’ and teleostean fishes is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009202030456

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The biochemistry of an acetylcholine receptor (1974)

Raftery, M. A. ; Vandlen, R. ; Michaelson, D. ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Supramolecular Structure 2 (1974), S. 582-592

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ISSN: 0091-7419

Keywords: Life Sciences ; Molecular Cell Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: The acetylcholine receptor from Torpedo californica electroplax has been studied at three levels of molecular organization: receptor-rich membrane fragments, solubilized and purified receptor, and reconstituted receptor in phospholipid vesicles. The binding of cholinergic ligands to the membrane-bound and the solubilized material is not cooperative, and the number of ligand sites is less than the number of toxin sites. In addition, the purified macromolecule contains the molecular features necessary for ion-translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified receptor and Torpedo phospholipids.

Additional Material: 10 Ill.