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  • 1
    ISSN: 1432-2242
    Keywords: Key words Intraspecific variation ; Interspecific variation ; Aegilops Sitopsis species ; RFLP analysis ; Nuclear DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Intraspecific variation ; Interspecific ; variation ; Aegilops Sitopsis species ; RFLPanalysis ; Nuclear DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation (π=0.061), which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Cultivated rice species ; Chloroplast DNA ; Mitochondrial DNA ; Nuclear DNA ; RFLP analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms of chloroplast (ct), mitochondrial (mt) and nuclear DNA were investigated using eight cultivars of Oryza sativa and two cultivars of O. glaberrima. Relative variability in the nuclear and cytoplasmic genomes was estimated by a common measure, genetic distance. Based on the average genetic distances among ten cultivars for each genome, the evolutionary variabilities of the mitochondrial and nuclear genomes were found to be almost the same, whereas the variability of the chloroplast genome was less than half that of the other two genomes. Cluster analyses on ct and mt DNA variations revealed that chloroplast and mitochondrial genomes were conservative within a taxon and that their differentiations were well-paralleled with respect to each other. For nuclear DNA variation, an array of different degrees of differentiation was observed in O. sativa, in contrast with little variation in O. glaberrima. As a whole, differentiation between O. sativa and O. glaberrima was clearly observed in all three genomes. In O. sativa, no notable difference was found between the cultivars ‘Japonica’ and ‘Javanica’, whereas a large differentiation was noticed between ‘Japonica’ (including ‘Javanica’) and ‘Indica’. In all three genomes, the average genetic distances within ‘Indica’ were much larger than those within ‘Japonica’ (including ‘Javanica’), and almost similar between ‘Japonica’ (including ‘Javanica’) and ‘Indica’. These facts indicate that differentiation in O. sativa was due mainly to ‘Indica’.
    Type of Medium: Electronic Resource
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