ISSN:
1573-7373
Keywords:
brain tumor
;
recombinant human tumor necrosis factor-α
;
blood flow
;
methionine uptake
;
autoradiography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The aim of the present study is tounderstand the therapeutic effects of recombinant human tumornecrosis factor-α (rH-TNF) on hemocirculation and metabolism ofbrain tumors. Using double-label autoradiographic technique, we havemonitored changes in regional cerebral blood flow (rCBF)and protein-bound fraction of (3H-methyl)-L-methionine, expressed as acid-insolublefraction (AIF), in rat brain tumors following treatmentwith intracarotid rH-TNF. The central portion of tumorsshowed a significant decrease in rCBF and AIFat 4 hours after the injection (p 〈0.01, p 〈 0.05, respectively, as compared withnon-treated control rats), turned microscopically necrotic at 24hours, and became more extensively necrotic at 72hours. Tumor cells remained viable only in theperipheral portion of the tumors after the treatment.The peripheral portion also showed a moderate decreasein rCBF, but less change in AIF to4 to 72 hours after the treatment. Neitheripsilateral nor contralateral non-involved cortex demonstrated appreciable changesin rCBF and AIF during the observed period.Intracarotid rH-TNF selectively reduces tumor rCBF and AIF,resulting in histological modification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005781210974
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