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  • 11
    Electronic Resource
    Electronic Resource
    BLT-esterase activity followingin vitro andin vivo activation of human lymphocytes with interleukin-2 (1991)
    Springer
    Biotherapy 3 (1991), S. 253-260 
    Details
    ISSN: 1573-8280
    Keywords: BLT-esterase ; cytotoxic cells ; immunotherapy ; interleukin-2 ; lymphokine-activated killer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract BLT-esterase and cytolytic activity by humanin vitro andin vivo generated Lymphokine Activated Killer (LAK) cells were measured. Lysates made from peripheral blood lymphocytes (PBL) of both normal donors and cancer patients receiving IL-2 therapy were assayed for BLT-esterase activity in a spectro-photometric assay. Cytotoxicity of PBL was measured in a51Cr-release assay. Both BLT-esterase activity and cytotoxicity increased when normal-donor PBL were stimulatedin vitro with IL-2, with greater activities at higher IL-2 concentrations. The activities also increased over time, peaking at 6 days ofin vitro stimulation. Patient PBL had increased BLT-esterase and cytotoxic activities after 4 weeks ofin vivo IL-2 treatment. This association of BLT-esterase activity and cytotoxicity with IL-2 activation is consistent with the model that LAK cytotoxicity is mediated by secretion of BLT-esterase associated cytolytic granules. Lymphocytes obtained afterin vivo IL-2 treatment and cultured for 3-4 hours in IL-2 show markedly augmented cytotoxic activity but no increase in their BLT-esterase activity. These results indicate that the increased cytotoxicity observed after this brief pulse ofin vitro IL-2 followingin vivo IL-2 treatment must result from effects of IL-2 other than the production of more esterase-containing cytolytic granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02171689
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    Strategies for improving antitumor activity utilizing IL-2: Preclinical models and analysis of antitumor activity of lymphocytes from patients receiving IL-2 (1992)
    Springer
    Biotherapy 4 (1992), S. 189-198 
    Details
    ISSN: 1573-8280
    Keywords: cytokines ; immunotherapy ; interleukin-2 ; interleukin-2 receptors ; lymphokine activated killer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusions Considerable enthusiasm remains for the successful utilization of the immune system for the immunotherapy of human cancers. Immunotherapeutic maneuvers have been able to mediate impressive antitumor responses for some patients with advanced and refractory malignancies. Unfortunately, the number of patients who benefit from current immunotherapies is low, while the toxicity for many of the patients receiving these treatments is high. It is becoming quite clear that the development of successful immunotherapeutic strategies will involve a carefully chosen combination of immunotherapeutic modalities or of immunotherapy combined with either surgery, radiation therapy, or chemotherapy. The use of an IL-2 based regimen which is clinically tolerable and can provide significant immune activation continues to remain central to many of these treatment approaches. Preclinicalin vitro and animal model systems can evaluate promising treatment strategies, including combination approaches. As an effective immunotherapeutic approach will likely require use of a combination of biologically active agents, the scheduling of these therapies may have profound importance both for optimal antitumor responses as well as clinical tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174205
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    Long-term maintenance of “cloned” human PLT cells in TCGF with LCL cells as a feeder layer (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 525-532 
    Details
    ISSN: 0091-7419
    Keywords: T lymphocytes ; HLA-D ; cloning ; TCGF ; PLT lymphocyte typing ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The long-term maintenance of T cells “cloned” by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 × 1012 cells from a “clone” seeded at one cell per well. Some of the clones tested express primed LD-typing activity.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130411
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    Paper (German National Licenses)
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