ISSN:
1573-8280
Keywords:
BLT-esterase
;
cytotoxic cells
;
immunotherapy
;
interleukin-2
;
lymphokine-activated killer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract BLT-esterase and cytolytic activity by humanin vitro andin vivo generated Lymphokine Activated Killer (LAK) cells were measured. Lysates made from peripheral blood lymphocytes (PBL) of both normal donors and cancer patients receiving IL-2 therapy were assayed for BLT-esterase activity in a spectro-photometric assay. Cytotoxicity of PBL was measured in a51Cr-release assay. Both BLT-esterase activity and cytotoxicity increased when normal-donor PBL were stimulatedin vitro with IL-2, with greater activities at higher IL-2 concentrations. The activities also increased over time, peaking at 6 days ofin vitro stimulation. Patient PBL had increased BLT-esterase and cytotoxic activities after 4 weeks ofin vivo IL-2 treatment. This association of BLT-esterase activity and cytotoxicity with IL-2 activation is consistent with the model that LAK cytotoxicity is mediated by secretion of BLT-esterase associated cytolytic granules. Lymphocytes obtained afterin vivo IL-2 treatment and cultured for 3-4 hours in IL-2 show markedly augmented cytotoxic activity but no increase in their BLT-esterase activity. These results indicate that the increased cytotoxicity observed after this brief pulse ofin vitro IL-2 followingin vivo IL-2 treatment must result from effects of IL-2 other than the production of more esterase-containing cytolytic granules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02171689
