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  • Electronic Resource  (3)
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  • Electronic Resource  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Interactions between Human Polymorphonuclear Leukocytes and Influenza Virus (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 22 (1985), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effects of influenza virus A (H3N2) on several functions of human polymorphonuclear leukocytes (PMN) were examined. Incubation of PMN with virus induced chemiluminescence, aggregation, and degranulation of the leukocytes. The amount of chemiluminescence generated increased from 1 × 106 to 6 × 106 cpm when 2.5 × 106 to 2 × 107 virus particles were added to 2.5 × 106 PMN. Maximal aggregation occurred within 2 min and the response depended on the amount of virus added to the PMN. Release of acid phosphatase by virus-treated PMN was 62±12% within 1 h compared with 7 + 7% by control PMN (P〈0.005). Incubation of PMN with influenza virus resulted in a diminished phagocytic activity of the phagocytes. PMN from a patient with chronic granulomatous disease were similarly affected. It was thus concluded that the observed defect in phagocytic activity was not due to the reactive oxygen species generated hy the PMN during incubation with virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1985.tb01935.x
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  • 2
    Electronic Resource
    Electronic Resource
    Human polymorphonuclear leukocytes release leukotriene B4 during phagocytosis ofStaphylococcus aureus (1986)
    Springer
    Inflammation 10 (1986), S. 37-47 
    Details
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We studied release of leukotriene B4 (LTB4) by human polymorphonuclear leukocytes (PMNs) during phagocytosis of staphylococci in the presence or absence of arachidonic acid. The 12×107 PMNs incubated with 3×109 opsonizedS. aureus and 50μM arachidonic acid released 1.45±0.42 nmol LTB4. No LTB4 was detected after stimulation of PMNs withS. aureus or arachidonic acid by themselves. However, by increasing the concentration of arachidonic acid to 200 or 400μM, 1.22±0.45 and 1.98±0.49 nmol LTB4, respectively, was released by PMNs. The effect of different bacteria-PMN ratios on LTB4 production was also studied. LTB4 varied from 0.3 to 2.0 nmol when bacteria/PMN ratios increased from 5 to 50 (respectively) in the presence of 50 μM arachidonic acid. Thus, phagocytizing PMNs produce LTB4 in the presence of arachidonic acid, and its production is dependent on the number of bacteria phagocytized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00916039
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of influenza virus on phagocytic cell function (1985)
    Springer
    Antonie van Leeuwenhoek 51 (1985), S. 509-510 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404505
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    Paper (German National Licenses)
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