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  • 1990-1994  (108)
  • 1860-1869  (4)
  • 1990  (108)
  • 1863  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 337 (1990), S. 41-43 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 44 (1990), S. 1-17 
    ISSN: 0730-2312
    Keywords: histone genes ; gene structure ; gene expression ; histone mRNA ; rat liver ; rat testis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A 6.86 kb rat genomic DNA fragment containing the testis-specific histone H1t gene and the histone H4t gene has been sequenced. S1-nuclease protection analyses of total cellular RNA from rat liver and testis showed that histone H1t mRNA was present only in testis. Examination of various highly enriched populations of rat testis cell types revealed that H1t mRNA was found exclusively in a fraction enriched in pachytene spermatocytes. When protein, DNA interactions within the proximal promoter region of the histone H1t gene were examined by electrophoretic mobility shift assays, only minor differences were found in mobility shift patterns of the H1t promoter in assays comparing binding of nuclear proteins from pachytene spermatocytes and early spermatids. However, major differences in binding were observed upon comparing nuclear proteins from rat pachytene spermatocytes to liver. Comparison of binding patterns of rat testis, rat hepatoma H4 cells, HeLa cells, and COS-1 cells also revealed dramatic differences. Transcriptional activity of the histone H1t promoter was examined by measuring H1t promoted chloramphenicol acetyltransferase (CAT) mRNA levels in transient experession assays in transfected rat hepatoma H4 cells, HeLa cells, and COS-1 cells. These assays revealed that the histone H1t promoted CAT gene functioned poorly in HeLa cells and COS-1 cells compared to expression with the parent SV40 promoted vector pSV2CAT. The H1t promoted CAT gene apparently did not work at all in transfected rat hepatoma H4 cells, which is consistent with testis germinal cell specific expression of the histone H1t gene.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 44 (1990), S. 177-187 
    ISSN: 0730-2312
    Keywords: translated mRNAs ; cytoskeleton ; HeLa cells ; signal peptide-histone fusion mRNA ; histone mRNA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Nearly all actively translated mRNAs are associated with the cytoskeleton in HeLa cells and the nature of this association is poorly understood. To gain insight into this association, we have examined and compared the cytoskeleton-mRNA interactions of a signal peptide-histone fusion mRNA (membrane-bound polysomal mRNA) to those of endogenous histone mRNA (nonmembrane-bound polysomal mRNA). We report here the detection of a cytoskeleton attachment site within the signal peptide-histone fusion mRNP/mRNA nucleotide sequence that is not present in wild-type histone mRNA or in HLA-B7 and chorionic gonadotropin-α membrane-bound polysomal mRNAs. These results support the possibility that there are multiple mechanisms for the attachment of specific classes of mRNAs to the cytoskeleton.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 144 (1990), S. 175-182 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Histone mRNAs are naturally localized on non-membrane-bound polysomes and selectively destabilized during inhibition of DNA replication. Targeting histone mRNA to membrane-bound polysomes, by incorporating sequences coding for a signal peptide into the message, results in the stabilization of the histone fusion mRNA when DNA synthesis is interrupted (Zambetti et al.: Proceedings of the National Academy of Sciences of the United States of America 84:2683-2687, 1987). A single nucleotide substitution that abolishes the synthesis of the signal peptide results in the localization of the histone fusion mRNA on non-membrane-bound polysomes to the same extent as endogenous histone mRNA and fully restores the coupling of histone fusion mRNA stability to DNA replication. Signal peptide-histone fusion mRNAs containing two point mutations that result in the incorporation of two positively charged amino acids into the hydrophobic domain of the signal peptide are partially retained on non-membrane-bound polysomes and are partially destabilized during inhibition of DNA synthesis. These data indicate that the degree to which the signal peptide-histone fusion mRNAs are associated with non-membrane-bound polysomes is correlated with the extent to which the mRNAs are degraded during inhibition of DNA synthesis. These results suggest that the subcellular location of histone mRNA plays an important role in the posttranscriptional regulation of histone gene expression.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 94 (1990), S. 3229-3230 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 94 (1990), S. 6943-6948 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 92 (1990), S. 1386-1396 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We presented some experimental results on flow small-angle light scattering ("flow SALS'') of lyotropic polymer liquid crystals which involved real-time, in situ, and simultaneous measurements of mechanical properties and small-angle light scattering (SALS) of a complex structured fluid. The flow-SALS studies were conducted under steady-state Couette flow and during relaxation process occurring after the cessation of the steady-state shear flow. We found the following universal behavior on the structure and properties for two kinds of the liquid crystal systems, i.e., poly(γ-benzyl L-glutamate) in m-cresol and hydroxypropyl cellulose in water. (i) Upon increasing shear rate γ(overdot), the "polydomain'' structure gradually tends to be transformed to the essentially "monodomain'' texture through annihilation of the disclinations. On the top of this general tendency, we found more specific characteristic of the fluid systems with γ(overdot), (ii) at γ(overdot)〈γ(overdot)c, a critical shear rate above which a remarkable shear thinning occurs, the disclinations are more or less uniformly distributed in space, while at γ(overdot)〉γ(overdot)c the disclinations are localized into domains and dispersed in the more or less uniform medium with a high degree of orientation, (iii) the relaxation after cessation of the steady state shear depends on its γ(overdot) before the cessation and is quite different below and above γ(overdot)c, and (iv) the cessation from γ(overdot)〉γ(overdot)c involves formation of the "band texture'' during the relaxation process of the fluids.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 93 (1990), S. 1442-1454 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The dissociative chemisorption probability of N2 on W(100) is found to proceed by way of two dynamically distinct channels. At low kinetic energies Ei, dissociation proceeds primarily by way of a precursor-mediated process, where the dissociation probability is found to fall with increasing Ei, reflecting the energy dependence of the trapping probability into this state. Dissociation at low energies is also strongly dependent on surface temperature Ts which effects the fraction of trapped species that desorb. For energies above about 0.45 eV, the dissociation probability is found to rise from a minimum of about 0.14 at Ts=800 K to over 0.45 at Ei=5 eV. Over this range we believe that kinetic energy enables the incident molecules to directly overcome a barrier in the reaction coordinate. Throughout the entire range of energies we observe only slight variations of the dissociation probability with the angle of incidence, with no discernible sensitivity for energies below ∼0.5 eV. For energies between 1 and 4 eV, associated with the "activated'' channel, we observe a slight preference for non-normal incidence, with a clear preference for normal incidence only for Ei〉5 eV.While the "precursor-mediated'' channel displays a considerable sensitively to surface temperature, results at high energy are found to be essentially independent of this parameter. Moreover, dissociation by way of the precursor-mediated channel is found to be insensitive to surface coverage, in contrast to a roughly linear decrease in the dissociation probability with surface coverage observed for dissociation via the activated process. In this latter case, we find that the saturation coverage remains approximately constant at about 0.6 atomic monolayers for all conditions, up to the highest incidence energies. This differs from previous observations for the dissociation of O2 and N2 on W(110), where the saturation coverage was found to rise with increasing Ei. Finally we find that the dissociation probability vs kinetic energy curve for the "direct'' dissociation case is qualitatively similar to that for the N2/W(110) system, but with a threshold that is ∼0.4 eV lower. We argue that the "precursor-mediated'' mechanism does not contribute significantly to dissociative chemisorption in the W(110) case and conclude that the primary difference between N2 dissociation on the W(110) and W(100) surfaces is that the barrier to dissociation is slightly higher in the W(110) case.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 92 (1990), S. 5622-5635 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Time-resolved fluorescence depolarization has been used to measure electronic excitation transport among naphthyl chromophores in polymeric glasses. 2-ethylnaphthalene randomly distributed in PMMA and 2-vinylnaphthalene/methyl methacrylate copolymer in PMMA were studied. It was found that excitation transport is dispersive at all temperatures studied, from 50 K to room temperature, i.e., the extent of transfer depends on the excitation wavelength within the S0–S1 absorption band. A theory based on the nondispersive, Förster mechanism for excitation transfer has been developed to describe dispersive transport. Good agreement between the theoretical and experimental results are achieved without resorting to adjustable parameters. Both the theory and experiment show that, for the observable used here, excitation at a certain wavelength, called the "magic wavelength,'' results in a time dependence that is identical to the Förster nondispersive result, i.e., dispersive transport appears to vanish.
    Type of Medium: Electronic Resource
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