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Isolation and characterisation of a bipotential haematopoietic cell line (1979)

DEXTER, T. M. ; ALLEN, T. D. ; SCOTT, D. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 277 (1979), S. 471-474

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Fig. 1 a, Cultured 416B cells stained with May-Grunwald Giemsa. Note large nuclear: cytoplasmic ratio, vacuolated cytoplasm (x750) b, Section through a pellet of 416B cells after glutaraldehyde-osmium fixation, showing the size variation of cultured cells. Three small round cells are apparent ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/277471a0

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Ultrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma (1979)

Allen, T. D. ; Iype, P. T.

Springer

Journal of cancer research and clinical oncology 95 (1979), S. 225-232

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ISSN: 1432-1335

Keywords: Cultured tumour cells ; Ultrastructure ; 3-D Colonies

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Cultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.