ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Ultrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma (1979)

Allen, T. D. ; Iype, P. T.

Springer

Journal of cancer research and clinical oncology 95 (1979), S. 225-232

add to mindlist on the mindlist

Details

ISSN: 1432-1335

Keywords: Cultured tumour cells ; Ultrastructure ; 3-D Colonies

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Cultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00410643

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Cultures from adult rat liver cells I. Establishment of monolayer cell-cultures from normal liver (1971)

Iype, P. T.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 78 (1971), S. 281-288

add to mindlist on the mindlist

Details

ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Establishment of monolayer cell-cultures from adult normal rat liver is described using cells isolated by a collagenase-hyaluronidase perfusion technique. The cells plated originally were polygonal in shape and contained a large number of mitochondria. However, cells with less granules were also observed in the primary culture, after a few days of plating. In subsequent subcultures, the latter type of cells could be grown continuously. After an initial lag, the cells attained a doubling time of 55 hours and a plating efficiency of 20%. The cells are “normal” with respect to their karyology, morphology and growth pattern. Difficulties encountered during the culture and the possible uses of these cultured cells in studies on carcinogenesis in vitro are discussed.

Additional Material: 6 Ill.