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Digitale Medien

A histofluorescent procedure for identifying marijuana cannabinoids (1983)

Bruni, A. ; Barni Comparini, I. ; Menziani Andreoli, E.

Springer

Cellular and molecular life sciences 39 (1983), S. 886-888

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ISSN: 1420-9071

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary A rapid and reliable fluorescence procedure is described as a test for the microscopical identification of the glandular hairs ofCannabis sativa. The proposed method, designated as the IFIM test (induced fluorescence identification for marijuana test), is based on the induction of a red fluorescence in cannabinoids by a hot clearing solution. The results, compared to those obtained by the classical RIM test, offer the possibility of more satisfactory identification of cannabis, hashish or marijuana in suspected samples.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01990420

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Digitale Medien

A method for localizing embryonal laticifers by combined conventional and fluorescence microscopy (1980)

Bruni, A. ; Tosi, Barbara

Springer

Protoplasma 102 (1980), S. 343-347

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ISSN: 1615-6102

Schlagwort(e): Double staining ; Euphorbiaceae ; Fluorescent staining ; Laticifers

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The authors describe a simple method based on malachite green and acid fuchsin for the detection of laticifers during the embryogenesis of someEuphorbiaceae plants by conventional and fluorescence microscopy. The strong sensitivity and specificity of the method make it suitable for the ontogenetic studies of laticifers. The results obtained are discussed in the context of the reactive mechanism of the staining and of the chemical composition of the embryonal laticifers.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01279597

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Digitale Medien

Two rapid fluorescence procedures for the detection of some thio pungent compounds in plant tissues (1980)

Bruni, A. ; Dall'olio, G.

Springer

Journal of molecular histology 12 (1980), S. 1-7

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ISSN: 1573-6865

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Two rapid flourescence procedures are described for detecting sulphydryl, disulphide and isothiocyanate groups of scented and pungent principles present in the vacuolar sap of onion, garlic and cabbage. To localize compounds containing sulphydryl groups, fresh or fixed frozen sections of the plants were treated with mercurochrome. After the fluorochromization, strongly-positive sulphydryl sites emitted an intense orange-red fluorescence, while weakly-positive sites emitted a distinctive red-brown fluorescence. Disulphide groups were detected by first reducing with thioglycolic acid to thiol groups before treating with mercurochrome. To effect isothiocyanate localization, frozen sections were exposed to ammonia: isothiocyanates were converted to thioureas and the engendered amino groups were revealed with fluorescamine.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01066532

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