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  • 1980-1984  (4)
  • 1950-1954
  • 1915-1919
  • 1890-1899
  • 1980  (4)
  • Life and Medical Sciences  (4)
  • 1
    Electronic Resource
    Electronic Resource
    The ultrastructure of oral (buccopharyngeal) membrane formation and rupture in the chick embryo (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 197 (1980), S. 441-470 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructure of the oral (buccopharyngeal) membrane was examined by transmission and scanning electron microscopy (SEM) from its initial formation (stage 8) to its complete disappearance (stage 20) in the chick embryo. Thinning of the oral membrane prior to rupture occurs in large measure by increased interdigitation between cells of the stomodeal ectoderm and foregut endoderm coincident with a decrease in the width of the intervening extracellular space. Large numbers of necrotic cells were not observed. Interdigitation of ectodermal and endodermal cells makes it increasingly difficult to discern two discrete epithelia, and no evidence that one germ layer disappears prior to the other was observed. Changes occurred in the fine structure of the extracellular matrix during formation and rupture of the oral membrane, and the organization of this material within the oral membrane differed from that in regions immediately lateral to it. Copious amounts of amorphous, flocculant (“lamina-like”) material are present within the oral membrane at all stages. The basal lamina of the ectoderm exhibits small loops or folds at early stages. These decrease in number as the basal lamina becomes discontinuous prior to establishment of direct intercellular contact between cells of the ectoderm and endoderm across the intervening extracellular compartment. Initial perforations of the oral membrane are preceeded by clefts between cells on both sides of this structure, and SEM observations suggest that cells of the oral membrane continue to interdigitate, elongate, and change relative positions during the rupture process.
    Additional Material: 39 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091970408
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Indirect immunofluorescent staining of fibronectin associated with the floor of the foregut during formation and rupture of the oral membrane in the chick embryo (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 198 (1980), S. 619-635 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution of the glycoprotein, fibronectin, within the cranial region of stage 8-16 chick embryos was examined by indirect immunofluorescence using paraffin sections exposed to affinity-purified rabbit anti-human CIG and FITC-conjugated goat anti-rabbit immunoglobulins. Fluorescence was present within the matrix surrounding the cranial mesenchyme, along the basal surfaces of all epithelia, and surrounding the notochord at all stages. Fluorescence associated with the floor of the foregut was particularly intense. The fluorescent layers beneath the ectoderm and endoderm of the oral (oropharyngeal) membrane at stage 8 merged into a single, continuous, intensely fluorescent line as the extra-cellular space within the oral membrane narrowed during stages 9-12. This line of uniform fluorescence parallels the previously described histological reorganization of the extracellular compartment of the oral membrane, but the ultrastructural localization of this fluorescent material remains unknown. Fluorescence was also intense beneath the foregut endoderm in the presumptive cardiac region caudal to the oral membrane and was continuous with strands of fluorescent material extending into the matrix of the dorsal mesocardium and cardiac jelly of the developing tubular heart. These observations indicate that the extracellular matrix associated with the floor of the entire foregut contains fibronectin during stages encompassing the formation and rupture of the oral membrane. The presence of fibronectin within the oral membrane and dorsal mesocardium, as well as between Rathke's pouch and infundibulum and within the closing plates between ectodermal clefts and endodermal pouches, is consistent with the possibility that this glycoprotein may play a role in adhesion at these sites.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091980407
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Glomerular architectural changes after a two-hour infusion of norepinephrine (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 159 (1980), S. 379-384 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Alterations in glomerular architecture have been suggested to play a role in the pathogenesis of several models of acute renal failure. In this study, mongrel dogs were subjected to an intrarenal infusion of norepinephrine for 2 hours. Light microscopy and scanning and transmission electron microscopy were used to describe glomerular architecture 2 days after the initial norepinephrine infusion. In addition, scanning electron microscopy was used to quantitate the percentage of “abnormal areas” in glomerular capillary-loop morphology in both norepinephrine-infused kidneys and the contralateral control kidneys. Alterations in glomerular structure in these experiments appeared to be much less extensive than previously reported. A variable amount of glomerular pedical shape simplification was seen, which involved about 15% of the capillary loop Quantitative evaluation revealed abnormal morphology of 15.2% ± 0.6% of the glomerular capillary loop in the norepinephrine-infused kidneys, compared to 2.9% ± 0.4 abnormal loop structure in the contralateral control kidneys (P 〈0.001). It is concluded that alterations in glomerular structure are not extensive in this model.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001590403
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A study of binding-solubilization of some glycolytic enzymes in striated muscle in situ (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 105 (1980), S. 409-416 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The solubilization of lactate dehydrogenase (LDH), glyceralde-hyde-3-phosphate dehydrogenase (GAPDH), and alpha-glycerophosphate dehydrogenase (aGPDH) was studied in pressed muscle as a function of ionic strength and NADH concentration.The results indicate that these factors affect the binding-solubilization of LDH and GAPDH in a similar way to their effect in dilute homogenized tissue. Alpha-glycerophosphate dehydrogenase was included as a typical soluble enzyme, since we have been unable to demonstrate its binding to subcellular fractions under any conditions. As with homogenized tissue, LDH was less susceptible to solubilization by ionic strength than GAPDH. It was demonstrated that LDH isozymes richer in heart-type subunits were more easily removed from muscle by centrifugation-imbibition than isozymes richer in the muscle-type subunits. This was interpreted as indicating that binding of the enzyme to subcellular structures was a major factor in the restricted removal of these enzymes from muscle, since only the muscle-type subunit is capable of binding to subcellular particles.It was further demonstrated that LDH could be taken up into muscle tissue, depleted in the enzyme, against an apparent concentration gradient. This was also interpreted as binding of the enzyme to the particulate structure of the muscle. Furthermore, this uptake of LDH occurred under conditions of ionic strength (0.25) and pH (7.5) that would prevent binding of the enzyme to the particulate fraction of a dilute suspension of homogenized muscle tissue. Thus, physiological conditions of pH and ionic strength do not necessarily induce solubilization of chicken breast muscle LDH in situ. Data obtained with dilute tissue homogenates, therefore, may not necessarily be easily and safely extrapolated to conditions in situ.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041050304
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    Paper (German National Licenses)
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