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Butyricin 7423 and the membrane H+-ATPase of Clostridium pasteurianum (1982)

Clarke, D. J. ; Kell, D. B. ; Morley, C. D. ; [et al.]

Springer

Archives of microbiology 131 (1982), S. 81-86

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ISSN: 1432-072X

Keywords: Bacteriocin ; Butyricin 7423 ; Clostridium pasteurianum ; Membrane H+-ATPase ; Adenosine triphosphatase

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The bacteriocin butyricin 7423 inhibited the activity of the membrane H+-ATPase (BF0, F1) of vegetative cells of Clostridium pasteurianum but not that of its soluble BF1 component. In vitro studies with the H+-ATPases of mutant strains selected for diminished sensitivity (a) to butyricin 7423 and (b) to dicyclohexylcarbodi-imide, confirmed that butyricin 7423 interacts with the BF0 component of this enzyme complex. Even so, certain other mutant strains displaying decreased sensitivity to butyricin 7423 possessed H+-ATPases which in vitro showed undiminished sensitivity to inhibition by the bacteriocin. Furthermore, from the changes in intracellular ATP concentration and in the rates and net extent of efflux of intracellular 86Rb+ ions that were provoked by exposure of the parent and several of the mutant strains to butyricin 7423, it was concluded that its primary bactericidal action was not attributable to stoichiometric inhibition of the membrane H+-ATPase. High extracellular concentrations of K+ ions enabled Cl. pasteurianum to survive exposure to low concentrations of this membrane-active bacteriocin.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00451503

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Effects of 60-Hz electric fields on specific humoral and cellular components of the immune system (1982)

Morris, J. E. ; Phillips, R. D.

New York, NY [u.a.] : Wiley-Blackwell

Bioelectromagnetics 3 (1982), S. 341-347

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ISSN: 0197-8462

Keywords: immunology ; mice ; 60-Hz electric fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Physics

Notes: We evaluated humoral and cellular functions of the immune system of Swiss-Webster mice exposed to 60-Hz electric fields at 100 kV/m. No significant differences were observed in primary antibody response to keyhole limpet hemocyanin (precipitating antibody levels) between exposed (30 or 60 days) and control mice, nor were there significant changes in mitogen-stimulation response of spleen cells from mice similarly exposed for 90 or 150 days when compared to sham-exposed animals.

Additional Material: 2 Ill.