Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
International journal of cosmetic science
7 (1985), S. 0
ISSN:
1468-2494
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
To assess ocular irritancy caused by chemical and cosmetic products a reliable method based on evaluation of histamine (Hm) in tears is presented. Hm is measured at picomole levels by HPLC and fluorimetric detection after fluorescamine-HM derivatization. In order to avoid any uncontrolled irritation and stimulation of the conjunctiva during sample collection, a procedure of conjunctiva lavage was developed. A balanced salt solution (50 μl) containing a known amount of Hm-fluorophore as reference standard is instilled in the conjunctiva fornix. After a few seconds 20 μl of tear fluid is collected: 10 μl are immediately analysed and 10 μl after derivatization reaction. In this way it is possible to evaluate tear dilution and to assess Hm content in less than 10 minutes.In a group of 20 normal subjects Hm has been determined in comparison with that of two volunteers after topical application of 50 μ of 0.2% and 0.4% sodium lauryl sulphate solution. A contact of 30 seconds of the cosmetic ingredient caused an immediate dose-dependent Hm release through a direct cytotoxic damage of cell membranes due to the surfactant action.Évaluation de l'irritation occulaire due aux cosmétiques par la détermination de l'histamine lacrimale
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1467-2494.1985.tb00389.x
Permalink
| Library |
Location |
Call Number |
Volume/Issue/Year |
Availability |
