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  • 1990-1994
  • 1985-1989  (2)
  • 1970-1974
  • 1989  (2)
  • Insulin receptor  (1)
  • Acetaldehyde
  • rice
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Years
  • 1990-1994
  • 1985-1989  (2)
  • 1970-1974
Year
  • 1
    ISSN: 1432-0428
    Keywords: Insulin receptor ; insulin proreceptor ; insulin resistance ; transformed lymphocytes ; point mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An alteration of an amino acid sequence in the processing site of the insulin proreceptor by a point mutation of the insulin receptor gene produced extreme insulin resistance. We characterized functional properties of the unprocessed insulin receptor in transformed lymphocytes from a patient. Insulin binding to intact cells and to a partially purified insulin receptor preparation was radically decreased to 20% and 18% of the control values, respectively. In competitive insulin binding to intact cells, [LeuA3]-, [LeuB24]-, [SerB24-insulin, and mini-proinsulin ([B(1–29)-Ala-Ala-Lys-A(1–21)]-insulin) had the same relative binding activity in both the patient's and the control cells, but proinsulin and IGF-I were markedly less able to displace 125I-insulin in the patient's cells. In contrast to the study in intact cells, proinsulin and IGF-I as well as other insulin analogues had the same relative binding activity to bind to the partially lectin-purified insulin receptor preparations from both the patient's and the control cells. As regards the signal transduction after receptor binding, insulin-stimulated autophosphorylation of the unprocessed insulin proreceptor occurred proportionally to the amount of decreased insulin binding. With 0.025% trypsin treatment, the abnormal binding characteristics and autophosphorylation were normalized through conversion to functionally normal receptors. In spite of the abnormal processing, self-association of receptors into oligomeric structures was observed in the proreceptor. These results suggest that the unprocessed insulin proreceptor in the plasma membranes has an altered conformation which affects its binding characteristics but not its intramolecular signal transmission.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 120 (1989), S. 165-170 
    ISSN: 1573-5036
    Keywords: Azolla ; phosphorus ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The trials to use Azolla as a green manure for rice culture were made in the Niger basin.Azolla pinnata (Niger isolate) was used for the experiments. The effect of phosphorus on the growth and N2-fixation was examined in the field and in the laboratory. The growth rate and N content were maximum with P 3.1 ppm culture solution under laboratory conditions. The threshold P content for the growth was 0.5–0.6% in the dry matter. Maximum N content was 4.1% in the laboratory culture. In the field culture, the effect of P fertilizer on the growth and N yield of Azolla was tested. The split application of 6.5 kg P ha−1 per 13 days was most effective in stimulating the growth of Azolla. One kg of P as triple superphosphate produced 3.66 kg N in the Azolla. Maximum growth rate and N content in the field trials was 4.3 days (doubling time) and 2.3%, respectively. The lower productivity in the field in comparison with the laboratory culture was considered to be due to higher temperature and light intensity. the growth of Azolla was suppressed in the hot season in the Niger basin. The growth rate and N content were reduced during the high temperature period over 30°C on an average. The effect of inoculation of Azolla on rice yield was tested in the field experiment. The grain yield was increased 27% by Azolla incolation over the treatment without Azolla inoculation in — N fertilizer treatments. While the growth of Azolla with rice plants did not attain saturated density (1.8 kg fresh weight m−2), the effect on the grain yield was comparable to 40 kg N ha−1 as urea.
    Type of Medium: Electronic Resource
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