ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Study was made of the effect of medium humidity, source and dose of nitrogen, dose of phosphorus, dose of inoculum and aeration on protein biosynthesis by strain Aspergillus oryzae A. or. 11 in solid state fermentation. It was found that for a maximal protein yield the medium ought to contain about: 60% of water, 36% of starchy raw materials d. m. (e.g. 28.8% of coarse rye meal and 7.2% of beet pulp), 0.85% of nitrogen sources (1.6% of (NH4)2SO4 and 1.1% of urea), and 0.35% of phosphorus source (1.3% of KH2PO4). pH of medium should be near 6.5. The dose of inoculum should not be lower than 108 spores/100 g medium.The duration of culture ought to be 20-22 h at 30-35°C, with aeration at least 40 dm3 of air/h × 1000 g medium. Under these conditions the protein yield is about 6.0-6.3 g/100 g of starting medium d.m. at the cost of utilization of about 25 g total carbohydrates.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370090107
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