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  • 1990-1994  (3)
  • 1991  (3)
  • 33.20.L  (1)
  • Chromatography, general  (1)
  • Intracellular Ca2+  (1)
  • Agrobacterium tumefaciens
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Years
  • 1990-1994  (3)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Microfluorimetric imaging study of the mechanism of activation of the Na+/H+ antiport by muscarinic agonist in rat mandibular acinar cells (1991)
    Springer
    Pflügers Archiv 419 (1991), S. 338-348 
    Details
    ISSN: 1432-2013
    Keywords: Na+/H+ exchange ; Intracellular pH regulation ; Salivary gland ; Protein kinase C ; Intracellular Ca2+ ; Acetylcholine ; Ionomycin ; Phorbol esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mechanism of regulation of intracellular pH (pHi) in dispersed acini from the rat mandibular salivary gland has been studied with a microfluorimetric imaging method and the pH probe 2′,7′-bis(2-carboxyethyl)-5(and −6)-carboxyfluorescein. The pHi in the TRIS/HEPES-buffered standard solution was 7.29±0.01. Addition of 1 μmol/l acetylcholine (ACh) or ionomycin caused a sustained increase in the pHi. These agents decreased pHi in the absence of external Na+ or in the presence of amiloride. The rate of pHi recovery from an acid load after NH 4 + prepulse was a linear function of pHi and increased as pHi became more acidic. Addition of ACh shifted the relationship towards a more alkaline pHi range. The increase in pHi induced by ACh or ionomycin was not inhibited by the protein kinase C inhibitors staurosporine (10 nM) and 1-(5-isoquinolinesulfonyl)-1-methylpiperazine (50 μmol/l). Addition of 0.1–1 μmol/l phorbol 12-myristate 13-acetate (TPA) had little effect on pHi within 10 min; however, exposure to TPA for 120 min resulted in a significant rise in pHi. In Ca2+-free solution with 50 μmol/l 8-(diethylamino)-octyl-3,4,5-trimethoxybenzoate, the ACh-induced rise in both pHi and cytosolic Ca2+ concentration was suppressed. ACh and ionomycin caused an increment of amiloride-sensitive acid output into the extracellular fluid, while 20 μmol/l 1-oleoyl-2-acetylglycerol had little effect on it. It was concluded that (a) stimulation with ACh activated the Na+/H+ antiport in the plasma membrane, (b) ACh also stimulated the intracellular acid production but acid extrusion by the Na+/H+ antiport prevented the cell from intracellular acidification, and (c) the major route of signal transduction for the ACh-induced activation of the Na+/H+ antiport was independent of protein kinase C but was dependent on the rise in cytosolic Ca2+ concentration. The implication of the cytosolic acidification and cell volume change in pHi regulation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371116
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Spectroscopic study on the crystal growth in gas evaporation: emission and absorption spectra from Cu smoke (1991)
    Springer
    The European physical journal 20 (1991), S. 403-405 
    Details
    ISSN: 1434-6079
    Keywords: 33.20.L ; 32.20.J ; 81.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Optical emission and absorption spectra from a growth zone in Cu smoke were measured by using a quartz prism spectrograph and a single-grating spectrophotometer. Atomic lines of Cu, band systems of Cu2 were observed. Two new band systems with 0–0 band heads at 252.88 and 238.79 nm were found. The continuous spectra due to Rayleigh scattering by fine particles were also observed. Variations of density of various species as a function of vertical position were revealed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01544022
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Information theory of optimization in chromatography: Optimum peak separation and dependency on peak area (1991)
    Springer
    Chromatographia 32 (1991), S. 233-237 
    Details
    ISSN: 1612-1112
    Keywords: Chromatography, general ; Optimization ; Information theory ; Kalman filter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary If a peak (j=2) moves along the time axis of a chromatogram with no change in the position τ1 of the other peak (j=1) or in areas A1 and A2, the optimum separation, Řs, is defined as the resolution Rs which provides the maximum information, called FUMI, among all possible positions τ2 of the peak (τ2≥τ1). This paper demonstrates that optimum separation, Řs, of chromatographic peaks critically depends on the peak areas. As the area ratio A2/A1 of overlapped peaks increases, greater separation (higher resolution Rs) is needed to obtain maximum information. The quantitative relationship between Řs and A2/A1 is derived by computer simulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02276246
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    Paper (German National Licenses)
    Fulltext
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