Bibliothek

X
feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
  • 1
    Digitale Medien
    Digitale Medien
    DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro (1993)
    Springer
    Archives of virology 131 (1993), S. 201-208 
    Details
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379092
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Cardiostimulatory action of coenzyme Q homologues on cultured myocardial cells and their biochemical mechanisms (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. S71 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Ubiquinone ; Cell culture ; Myocardium ; ATP ; Isoproterenol ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The effect of coenzyme Q (CoQ) homologues on the beating of myocardial cells was investigated in cultured cell sheets from mouse fetuses and quail embryos. Myocardial cell sheets grown in Eagle's minimum essential medium with fetal bovine serum showed very weak and irregular beating when this serum was removed from the medium. However, the depressed beating rate and amplitude recovered almost completely within a few minutes by adding CoQ10 to the medium, and the effect of Co10 continued over 1 h. CoQ9 showed a cardiostimulatory effect similar to that of CoQ10 but CoQ8 and COQ7 showed almost no effect. Short homologues (less than CoQ4) inhibited the beating of cell sheets. The cardiostimulatory effect of CoQ10 was not blocked by atenolol, a selective β-blocker. In addition, CoQ10 stimulated the formation of ATP, not cAMP. CoQ0 and COQ3 inhibited beating rates by inhibiting ATP formation. In conclusion, only native CoQ homologues having a nona- or decaprenyl group showed a cardiostimulatory effect on cultured myocardial cells, probably by stimulating mitochondrial ATP formation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226844
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    The role of monoclonal antibody A7 as a drug modifier in cancer therapy (1993)
    Springer
    Cancer immunology immunotherapy 36 (1993), S. 177-184 
    Details
    ISSN: 1432-0851
    Schlagwort(e): Monoclonal antibody ; Drug modifier ; Cancer chemotherapy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An anticancer antibiotic, neocarzinostatin (NCS), was covalently conjugated to the murine monoclonal antibody A7 (mAb A7), which recognizes the glycoprotein on the cell surface of human colon cancer. The biological and pharmacological properties of the conjugate (A7-NCS) were examined and compared with those of unconjugated NCS. A7-NCS exhibited a strong binding and cytotoxicity to the cell and an antigen-specific tumor accumulation. Significant tumoricidal effects in vivo were observed in the antigen-positive tumor-bearing mice treated with A7-NCS, whereas NCS mixed with mAb A7 and NCS alone were relatively ineffective. In the antigennegative tumor, the tumoricidal effect of A7-NCS was lower than in the antigen-positive tumor. The NCS concentration in blood and tumor were significantly elevated by conjugation to mAb A7. The NCS localization in tumor was higher in the antigen-positive tumor than in the antigen-negative tumor. Death due to acute toxicity was observed at a dose of 20 units (U) NCS in mice treated with unconjugated NCS, whereas toxicity was seen with a much higher dose of NCS (100 U) if the drug was conjugated to the mAb. These findings show that mAb A7 confers more favorable pharmacological properties on an anticancer drug, making it potentially more useful for cancer chemotherapy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01741089
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    High resolution detection of x-rays with a Nb-based STJ detector (1993)
    Springer
    Journal of low temperature physics 93 (1993), S. 567-572 
    Details
    ISSN: 1573-7357
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A high energy-resolution of 88 eV has been achieved for 5.9-keV x-rays with a large area (178×178 µm2) Nb/Al-AlOx/Al/Nb superconducting tunnel junction (STJ) detector, which is stable at room temperature and robust to thermal cycles. The energy resolution is higher than those of semiconductor detectors. The resolution and the short shaping-time-constant, 2 µs, of the main amplifier used to obtain the energy resolution indicate that STJ detectors can be developed as nuclear radiation detectors with high energy resolution even for high rate radiations. Besides, a theoretical limit of energy resolution due to the statistical fluctuation of signal charges is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00693477
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 5
    Digitale Medien
    Digitale Medien
    PCNA-binding to DNA at the G1/S transition in proliferating cells of the developing cerebral wall (1993)
    Springer
    Journal of neurocytology 22 (1993), S. 1096-1102 
    Details
    ISSN: 1573-7381
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Proliferating cell nuclear antigen is a nuclear protein essential to DNA synthesis in eukaryotic cells. It is known to form part of a multi-protein complex which binds to DNA from the outset of S-phase of the cell cycle. We define in this analysis the interval of proliferating cell nuclear antigen binding to DNA (strictly speaking, the interval through which proliferating cell nuclear antigen is stained immunohistochemically after ethanol fixation) with respect to the stages of the cell cycle in the intact mammalian brain. The epithelium of the developing cerebral wall is favourable for such an analysis because nuclei at the same stage of the cell division cycle are spatially aligned with each other at the same depth of the epithelium. Therefore spatial location of a nucleus within the epithelium is a reliable indicator of the stage of the cell cycle for that nucleus. Proliferating cell nuclear antigen-DNA binding in this epithelium is initiated in the final 5% (26 min) of G1-phase and continues through the initial 35% (1.3 h) of S-phase. This phasic pattern of proliferating cell nuclear antigen-DNA binding, as revealed for the first time in the intact cerebral wall, approximates closely the phasic pattern as it has been characterized until now onlyin vitro in vertebrate cell lines. This analysis illustrates the potential of the cerebral proliferative epithelium for study of the molecular events of the cell cycle underin vivo conditions of histogenetic regulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01235751
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 6
    Digitale Medien
    Digitale Medien
    Chromosomal location and structure of amplicons in two human cell lines with coamplification of c-myc and Ki-ras oncogenes (1993)
    Springer
    Somatic cell and molecular genetics 19 (1993), S. 21-28 
    Details
    ISSN: 1572-9931
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Gene amplification is a major mechanism through which oncogenes and genes responsible for drug resistance are overexpressed in neoplastic cells, and several models for structure of amplified units (amplicons) are postulated. In order to identify consistent changes associated with oncogene amplification, we analyzed chromosomal location and physical distance of amplicons of two independent human cell lines that have coamplified c-myc and Ki-ras oncogenes. In one cell line, KHC287, amplified c-myc genes were localized in two chromosomes and Ki-ras in three chromosomes. One marker chromosome was almost entirely encompassed by both amplified genes. In the other cell line, Lu-65, both of the amplified genes shared the same locus, on chromosome 12q+. The two genes, however, are more than 1500 kb apart in both cell lines. The above findings indicate that two different amplified genes became associated on one chromosome in two independent cell lines. This suggests that a common mechanism is associated with chromosomal rearrangements affecting different amplified genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01233951
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 7
    Digitale Medien
    Digitale Medien
    Langmuir-Blodgett films of polymethacrylates with side chains containing functionalized aromatic schiff's bases (1993)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 31 (1993), S. 165-173 
    Details
    ISSN: 0887-6266
    Schlagwort(e): Langmuir-Blodgett film formation by polymethacrylates with nonlinear optical side chains ; polymethacrylate films by Langmuir-Blodgett technique, configuration of nonlinear optical substituents in ; orientation of nonlinear optical moieties in Langmuir-Blodgett films of substituted polymethacrylates ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Langmuir-Blodgett (LB) film formation has been investigated for polymers in which non-linear optical (NLO) moieties are attached to the side chain of a polymethacrylate (PMA) backbone. Polymer monolayers were successfully transferred onto hydrophilic glass substrates using a moving-wall type LB trough. The LB films were characterized by pressure-area isotherms, polarized ultraviolet visible spectra, ellipsometry, and second harmonic generation measurements. The characterization shows that NLO moieties in the side chain are inclined at a shallow angle to the plane of the substrate. The second-order nonlinear optical coefficient and molecular hyperpolarizability were also calculated. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/polb.1993.090310205
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...