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Detection ofBorrelia burgdorferi by nested polymerase chain reaction in cerebrospinal fluid and urine of children with neuroborreliosis (1993)

Huppertz, H. -I. ; Schmidt, H. ; Karch, H.

Springer

European journal of pediatrics 152 (1993), S. 414-417

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ISSN: 1432-1076

Keywords: Neuroborreliosis ; Borrelia burgdorferi ; Polymerase chain reaction

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Diagnosis of neuroborreliosis is often difficult since history and clinical presentation may be non-specific and serological tests may initially be negative. We therefore tested the polymerase chain reaction (PCR) for the detection of borrelial sequences in CSF and urine samples of consecutive children with neuroborreliosis seen in a single summer season. Four of eight children were negative in serum for antibodies toBorrelia burgdorferi. Two of eight children were PCR-positive in CSF and one other child was positive in urine. In two out of four children PCR was the only laboratory test confirming the clinical diagnosis. All children recovered after treatment with third generation cephalosporins. When seven of eight children were re-examined 6 months later all were healthy and antibodies toB. burgdorferi were detected in their serum. PCR may assist the paediatrician in establishing a diagnosis of neuroborreliosis; however, a negative result does not rule out neuroborreliosis. PCR is an adjunct, but no substitute for clinical judgement and serology.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01955900

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Excimer laser micromachining (1993)

Ihlemann, J. ; Schmidt, H. ; Wolff-Rottke, B.

New York, NY [u.a.] : Wiley-Blackwell

Advanced Materials for Optics and Electronics 2 (1993), S. 87-92

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ISSN: 1057-9257

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Electrical Engineering, Measurement and Control Technology , Physics

Notes: The machining of several materials such as polymers, metals and ceramics using excimer lasers (193, 248 and 308 nm) was investigated. By photoablation, micrometer resolution can be achieved for polymers if the wavelength and fluence are chosen properly. High-definition processing of metals is complicated by the occurrence of surface melting. An interaction between the ablation plume and the walls of the laser-drilled holes leads to hole widening in the case of polymers at high fluences. For small structures (〈100 μm) all materials investigated show a size-dependent ablation rate. This effect has to be taken into account if a precise ablation depth is required for a pattern with structures of different sizes.

Additional Material: 4 Ill.