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  • 1
    Electronic Resource
    Electronic Resource
    Mapping salt-tolerance genes in tomato (Lycopersicon esculentum) using trait-based marker analysis (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 184-192 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Salt tolerance ; Seed germination ; Isozyme markers ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The germination responsiveness of an F2 population derived from the cross Lycopersicon esculentum (UCT5) x L. pennellii (LA716) was evaluated for salt tolerance at two stress levels, 150 mM NaCl + 15 mM CaCl2 and 200 mM NaCl + 20 mM CaCl2. Individuals were selected at both tails of the response distribution. The salt-tolerant and salt-sensitive individuals were genotyped at 16 isozyme loci located on 9 of the 12 tomato chromosomes. In addition, an unselected (control) F2 population was genotyped at the same marker loci, and gene frequencies were estimated in both selected and unselected populations. Trait-based marker analysis was effective in identifying genomic locations (quantitative trait loci, QTLs) affecting salt tolerance in the tomato. Three genomic locations marked by Est-3 on chromosome 1, Prx-7 on chromosome 3, and 6Pgdh-2 and Pgi-1 on chromosome 12 showed significant positive effects, while 2 locations associated with Got-2 on chromosome 7 and Aps-2 on chromosome 8 showed significant negative effects. The identification of genomic locations with both positive and negative effects on this trait suggests the likelihood of recovering transgressive segregants in progeny derived from these parental lines. Similar genomic locations were identified when selection was made either for salt tolerance or salt sensitivity and at both salt-stress treatments. Comparable results were obtained in uni- and bidirectional selection experiments. However, when marker allele gene frequencies in a control population are unknown, bidirectional selection may be more efficient than unidirectional selection in identifying marker-QTL associations. Results from this study are discussed in relationship to the use of molecular markers in developing salt-tolerant tomatoes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223763
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  • 2
    Electronic Resource
    Electronic Resource
    Relationship between safety data and biocontainment design in the environmental assessment of fermentation organisms — An FDA perspective (1993)
    Springer
    Journal of industrial microbiology and biotechnology 11 (1993), S. 217-222 
    Details
    ISSN: 1476-5535
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The Center for Veterinary Medicine requires strain/construct-specific data for recombinant fermentation organisms used in the production of animal drugs and feed additives. Fermentation plant biocontainment schemes are chosen based, in part, upon the ability of the organism to survice and persist in the environment and to transfer genetic information to indigenous organisms. Survival and persistence study methods may include one of the following ecosystems: activated sludge, mammalian gut, soil or river water. Gene transfer protocols can be incorporated into a persistence study. These studies are designed to show that the recombinant construct behaves similarly to the host in a representative ecosystem where the organism could be introduced inadvertently. The studies need to provide repeatable results and reflect current state-of-art design and methods. Data verification is conducted by FDA investigators during Good Laboratory Practice inspections. Biocontainment guidelines, such as those developed by the NIH Recombinant DNA Advisory Committee, set general biocontainment goals for large groupings of recombinant organisms. The FDA, as required under the National Environmental Policy Act, must base its decision making on verifiable scientific data specific to each application. Therefore, in addition to using these guidelines as benchmarks, sponsors are required to submit strain/construct-specific data to support the selection of an appropriate biocontainment level. Once additional well-controlled studies for a variety of constructs are available, broader generalizations as to biocontainment may be drawn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569594
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  • 3
    Electronic Resource
    Electronic Resource
    Radiation-induced crosslinking of polyethylene in the presence of acetylene: A gel fraction, UV-visible, and ESR spectroscopy study (1993)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 31 (1993), S. 807-819 
    Details
    ISSN: 0887-6266
    Keywords: polyethylene, polyene formation on irradiation with acetylene present in ; polyene formation in irradiation of polyethylene with acetylene present ; crosslinking in polyethylene irradiated with acetylene present ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Electron-spin resonance (ESR) and altraviolet (UV) visible spectroscopic evidence has been found for the formation of diene, triene and tetraene, following the irradiation of polyethylene in the presence of acetylene. The polyenes are formed by a mechanism which is different from that observed under vacuum or with inert gas saturation. The sum of the G (polyene) values obtained by UV spectroscopy is almost half that of initial radical formation. It is concluded that polyene bridges, predominantly diene, form crosslinks between radical pairs. G (X) values determined from gel fraction data, using Saito-Kang-Dole theory, are found to be greatly in error and misrepresentative of crosslink changes. © 1993 John Wiley & Sons, Inc.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1993.090310709
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    Paper (German National Licenses)
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