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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing (1995)
    Springer
    Diabetologia 38 (1995), S. 381-386 
    Details
    ISSN: 1432-0428
    Keywords: Random cDNA sequencing ; MIN6 ; pancreatic islet beta cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To understand the molecular basis of glucose concentration-responsive insulin synthesis and secretion from pancreatic islet beta cells, a group of pancreatic islet beta-cell-related cDNAs was cloned. A pair of cDNA libraries was constructed from a mouse pancreatic islet beta-cell line of MIN6, which was cultured in either high glucose or low glucose media. By applying a random cDNA sequencing approach, 503 and 395 independent species were obtained from a total of 1,011 and 762 clones in the high glucose and low glucose library, respectively. The unknown genes comprised the majority of about 70% independent clones in both libraries. In Northern blot analysis, 311 (69.4%) of 448 independent clones showed positive signals within 72 h of autoradiographic exposure. Surprisingly, 150 (48.2%) out of 311 positive clones showed positive signals to MIN6 cells, but not to NIH/3T3 fibroblasts. The expression level of three unknown clones were glucose-concentration dependent. Combination of a random cDNA sequencing approach and Northern blot analysis is useful to obtain a large number of novel genes and islet beta-cell-related genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410274
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing (1995)
    Springer
    Diabetologia 38 (1995), S. 381-386 
    Details
    ISSN: 1432-0428
    Keywords: Key words Random cDNA sequencing ; MIN6 ; pancreatic islet beta cell.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To understand the molecular basis of glucose concentration-responsive insulin synthesis and secretion from pancreatic islet beta cells, a group of pancreatic islet beta-cell-related cDNAs was cloned. A pair of cDNA libraries was constructed from a mouse pancreatic islet beta-cell line of MIN6, which was cultured in either high glucose or low glucose media. By applying a random cDNA sequencing approach, 503 and 395 independent species were obtained from a total of 1,011 and 762 clones in the high glucose and low glucose library, respectively. The unknown genes comprised the majority of about 70 % independent clones in both libraries. In Northern blot analysis, 311 (69.4 %) of 448 independent clones showed positive signals within 72 h of autoradiographic exposure. Surprisingly, 150 (48.2 %) out of 311 positive clones showed positive signals to MIN6 cells, but not to NIH/3T3 fibroblasts. The expression level of three unknown clones were glucose-concentration dependent. Combination of a random cDNA sequencing approach and Northern blot analysis is useful to obtain a large number of novel genes and islet beta-cell-related genes. [Diabetologia (1995) 38: 381–386]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410274
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A note on the chemical composition of lake water in the Laguna Amarga, a saline lake in Patagonia, Chile (1995)
    Springer
    International journal of salt lake research 4 (1995), S. 165-167 
    Details
    ISSN: 1573-8590
    Keywords: chemistry ; saline ; lake ; Laguna Amarga ; Patagonia ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geography
    Notes: Abstract The chemical composition of lake water in Laguna Amarga, a small, shallow, saline lake near the Torres del Paine National Park (at 51°S), Chilean Patagonia, was studied in January, 1993. The water was strongly alkaline (pH 9.4) conductivity was 71.4 mS cm−1, and salinity was 77 g L−1. The major ions were sodium and sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990801
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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