ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Manganese peroxidases were overproduced by Phanerochaete chrysosporium I-1512 immobilized on nylon net in a bubble-column reactor. This study investigates a new design of bioreactor, a compromise between a pneumatic reactor and an immobilized biofilm reactor. The carrier, a sheet of nylon net, was maintained by a cylindrical stainless-steel frame installed vertically. It was characterized by its hydrophilic nature, its surface morphology and its surface roughness. P. chrysosporium adhesion was highly efficient; mycelial hyphae invaded the tridimensional structure and strengthened the bonding to the network, as shown by electron scanning microscopy. High levels of Mn peroxidases were produced by strain I-1512 under conditions of glycerol and nitrogen sufficiency when the medium was supplemented with phospholipid and veratryl alcohol. Yields of 3600 U/l Mn peroxidase were produced after 95 h of incubation, indicating significant productivity for industrial purposes (900 U day-1 l-1).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00178608
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