ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The production of a highly thermostable mannanase by Rhodothermus marinus was increased 16.5-fold by optimising the concentrations of locust bean gum and yeast extract using central composite designs. The optimised medium and culture conditions yielded mannanase activity at 495 nkat ml−1 (248 nkat mg−1 protein). In addition, α-L-arabinofuranosidase, β-xylanase, β-xylosidase, β-glucosidase, β-mannosidase, α-galactosidase, β-galactosidase and endoglucanase activities were detected at 32 nkat ml−1, 30 nkat ml−1, 16 nkat ml−1, 15 nkat ml−1, 0.1 nkat ml−1, 1 nkat ml−1, 0.5 nkat ml−1 and 8 nkat ml−1, respectively. No filter paper cellulase activity could be detected. The optimum pH of the mannanase was 5.0–6.5 and it showed high stability from pH 5 to 10 after 16 h incubation at 50 °C. The enzyme activity was maximum at 85 °C, with half lives of 45.3 h at 85 °C and 4.2 h at 90 °C. This is the first report on the production of such a high activity of extremely thermostable mannanase by an extreme thermophilic bacterium. © Rapid Science Ltd. 1998
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005330618613
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