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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 85 (1999), S. 2441-2447 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The operational lifetime of polymer light-emitting diodes was studied at several temperatures in the range from 25 to 85 °C. When operated in constant current mode, at luminances greater than 100 cd/m 2, lifetimes of around 20 000 h were noted. Two significant changes in performance were found during continuous operation: the luminance of the devices varied in a nonmonotonic fashion, and the operating voltage increased in a linear fashion. Both of these changes were thermally activated, with the changes accelerated at higher temperatures. These changes are also accelerated at higher current densities. We discuss possible mechanisms for these degradation processes. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Key words Liver ; Mucin ; Thomsen-Friedenreich-related antigens ; Carbohydrates ; Cytokeratin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The expression of epithelial mucins and Thomsen-Friedenreich-related antigens in preneoplastic and neoplastic hepatocellular lesions was systematically investigated using an in situ immunohistochemical staining approach. MUC1, MUC2, TF, sialosyl-TF, Tn, sialosyl-Tn, α2,3-linked sialic acid, and α2,6-linked sialic acid were examined in normal and cirrhotic human liver and in human hepatocellular carcinomas (HCCs) and cholangiocarcinomas. Normal hepatocytes and preneoplastic foci of altered hepatocytes did not express MUC1, MUC2, TF, Tn, s-Tn, or α2,6-linked sialic acid. In contrast, HCCs showed positive reactions for MUC1, TF, Tn, s-Tn, and α2,6-linked sialic acid. MUC2 was absent in normal biliary epithelial cells, but present in cholangiocarcinomas. The staining of MUC1, or s-Tn and α2,6-linked sialic acid in human normal liver tissues and various liver diseases did not change after specific treatments such as periodate oxidation or saponification, indicating that their expression in HCC does not result from incomplete glycosylation or low O-acetylation, respectively. MUC1, TF, Tn, s-Tn, and α2,6-linked sialic acid may be useful as indicators of progression of HCC in tissue sections, and perhaps also as targets for diagnostic and therapeutic approaches in vivo.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2013
    Keywords: Key words Anti TGF-β receptor antibody ; Heat shock protein 70 ; Human skin fibroblast cell ; Transforming growth factor-β ; Ultraviolet B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Ultraviolet B (UVB) alters the expression of heat shock protein 70 (HSP70) in cultured fibroblast cells derived from human skin. However, the nature of the signal transduction pathway remains to be determined. Transforming growth factor-β (TGF-β) has a large variety of biological functions, including cell growth control, modulation of inflammation and immunoregulation. In this study, we examined whether TGF-β is associated with the process of HSP70 expression induced by UVB irradiation. The constitutive expression of TGF-β1 mRNA and HSP70 expression in human skin fibroblast cells were detected using reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. The results indicate that: (1) UVB irradiation stimulates HSP70 expression in a dose- and time-dependent manner, (2) constitutive expression of TGF-β1 mRNA is detected after UVB irradiation, the level of which peaks at 4 h after 10 mJ cm–2 of UVB irradiation, (3) HSP70 expression is induced by TGF-β1 without UVB irradiation, and (4) HSP70 expression induction with UVB irradiation is inhibited by preincubation of the cells with the anti-TGF-β type II receptor antibody. Our results suggest that HSP70 expression induced by UVB involves the autocrine signalling of TGF-β production.
    Type of Medium: Electronic Resource
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