ISSN:
1569-8041
Schlagwort(e):
DPD
;
5-fluorouracil
;
immunohistochemistry
;
RT–PCR
;
Western blotting
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract Background: Dihydropyrimidine dehydrogenase (DPD) is the firstenzyme that metabolizes 5-fluorouracil (5-FU). Until now, enzymatic activityor mRNA expression of DPD has been investigated. However, there are no paperson immunohistochemical evaluation of DPD. We investigated DPD staining onimmunohistochemistry, and examined the relationship among immunohistochemicalscore, protein level and mRNA expression of DPD. Materials and methods: Forty-seven resected colon cancerspecimens, four colon cancer cell lines, two xenografts by colon cancer celllines, and human mononuclear cells were used. Immunohistochemistry wasperformed using DPD monoclonal antibody. Protein levels were determined byWestern blot analysis. And mRNA levels were calculated by semi-quantitativereverse transcription polymerase chain reaction (RT–PCR). Results: DPD was strongly expressed in the cytoplasm of cancercells, and in the cytoplasm of macrophage and plasma cells. Theimmunohistochemical score was more correlated with protein levels (P= 0.0054) than mRNA expression (P = 0.9028). Conclusions: We investigated the characterization of DPDimmunohistochemically, and showed that immunohistochemical expression of DPDcan be used to predict the sensitivity of colorectal carcinomas to 5-FU.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008337913456
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