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  • 2000-2004  (2)
  • 1980-1984
  • 1965-1969
  • 2004  (2)
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  • 2000-2004  (2)
  • 1980-1984
  • 1965-1969
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  • 1
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Using the sera from buckwheat (BW)-allergic patients, several putative causative molecules were reported. However, few molecules were determined on the molecular structure. We demonstrated in 2000 that the major allergen with 24 kDa (BW24KD) is a legumin-like storage protein.Objective:  The aim of this study was to isolate and characterize further a major allergen with 10 kDa by molecular cloning.Methods and results:  Buckwheat allergens were identified by immunoblotting analysis using sera from 14 allergic and two nonallergic individuals. We identified a protein with 10 kDa (BW10KD) that reacted with immunoglobulin E (IgE) more strongly than with IgG and IgA in 57% of the allergic patients but not with IgE in nonallergic individuals. Analyses were performed by N-terminal amino acid sequencing and molecular cloning. Physiological significance was assessed by an immunoblotting experiment showing that the reactivity of an allergic patient's serum IgE to BW10KD was competitively inhibited by natural BW extracts.Conclusion:  Molecular cloning experiments indicated that BW10KD as a BW allergen was a member of the 2S-albumin multigene family.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    PO Box 1354, 9600 Garsington Road, Oxford OX4 2XG, UK. : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 27 (2004), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Flaking type failure in rolling-contact processes is usually attributed to fatigue-induced subsurface shearing stress caused by the contact loading. Assuming such crack growth is due to mode II loading and that mode I growth is suppressed due to the compressive stress field arising from the contact stress, we developed a new testing apparatus for mode II fatigue crack growth. Although the apparatus is, as a former apparatus was, based on the principle that the static KI mode and the compressive stress parallel to the pre-crack are superimposed on the mode II loading system, we employ direct loading in the new apparatus. Instead of the simple four-point-shear-loading system used in the former apparatus, a new device for the application of a compressive stress parallel to the pre-crack has been developed. Due to these alterations, mode II cyclic loading tests for hard steels have become possible for arbitrary stress ratios, including fully reversed loading (R=−1); which is the case of rolling-contact fatigue. The test results obtained using the newly developed apparatus on specimens made from bearing steel SUJ2 and also a 0.75% carbon steel, are shown.
    Type of Medium: Electronic Resource
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