ISSN:
1432-1017
Keywords:
Surface-enhanced resonance Raman scattering (SERRS)
;
Intact cells
;
Erythrocyte
;
Membrane proteins
;
Bio-engineering
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Semi-quantitative and direct determination of labelled sulphydryl groups on the surface of intact erythrocytes has been accomplished for the first time with surface-enhanced resonance Raman scattering (SERRS). The method, which involves the use of citrate-reduced silver colloids, is sensitive and selective. A 10−8 M effective concentration of picomole quantities of sulphydryl groups was determined in the presence of the normally overwhelming signal from haemoglobin. This seminal study suggests that SERRS may be applied to other in situ, site-directed labelling experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00185120
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