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Direct spectroscopic determination of functional sulphydryl groups on intact cell surfaces by surface-enhanced resonance Raman scattering (1992)

Rospendowski, B. N. ; Campbell, J. M. ; Reglinski, J. ; [et al.]

Springer

European biophysics journal 21 (1992), S. 257-261

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ISSN: 1432-1017

Keywords: Surface-enhanced resonance Raman scattering (SERRS) ; Intact cells ; Erythrocyte ; Membrane proteins ; Bio-engineering

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Physics

Notes: Abstract Semi-quantitative and direct determination of labelled sulphydryl groups on the surface of intact erythrocytes has been accomplished for the first time with surface-enhanced resonance Raman scattering (SERRS). The method, which involves the use of citrate-reduced silver colloids, is sensitive and selective. A 10−8 M effective concentration of picomole quantities of sulphydryl groups was determined in the presence of the normally overwhelming signal from haemoglobin. This seminal study suggests that SERRS may be applied to other in situ, site-directed labelling experiments.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00185120

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Molecular recognition using surface-enhanced Raman scattering from citrate-reduced silver colloids in non-aqueous solvents (1991)

Clarkson, J. ; Campbell, C. ; Rospendowski, B. N. ; [et al.]

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 22 (1991), S. 771-775

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ISSN: 0377-0486

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: Surface-enhanced Raman scattering (SERS) from citrate-coated colloidal particles suspended in organic solvents is used to study surface reactions in an organic phase. Stable colloids which gave good SERS were produced from ethanol and acetonitrile. SERS was easily discriminated from the bulk solvent Raman scattering. Compounds which are sparingly soluble in water including 2,2′-bipyridine and benzene-1,2,4,5-tetracarboxylic acid dianhydride produced good SERS in ethanol. In the latter case, no SERS was observed from an aqueous colloid. Therefore, some form of solvent-dependent molecular recognition may operate. The formation of a bipyridine complex with copper(II) at the surface in a solvent system in which the ligand and complex are both soluble was observed. The solvent altered surface reactions. In the ethanol colloid, in contrast to the result in aqueous colloid, pyridine does not replace citrate readily. This work demonstrates the potential for the expansion of the use of colloidal SERS into the field of surface reactions in contact with an organic phase and is related to metal liquid-like films.

Additional Material: 4 Ill.