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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Earth, moon and planets 66 (1994), S. 29-30 
    ISSN: 1573-0794
    Schlagwort(e): Comet Shoemaker-Levy 9 ; Jupiter ; albedo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract The impact sites of the Comet Shoemaker-Levy 9 are observed with the 101-cm telescope at Bisei Astronomical Observatory until the end of August with B, V, R, I, and methane-band (0.89εm) filters. The outline of the observation is briefly described.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Archives of dermatological research 284 (1992), S. 307-308 
    ISSN: 1432-069X
    Schlagwort(e): Calmodulin ; Normal human epidermis ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 48 (1992), S. 384-385 
    ISSN: 1600-5759
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 51 (1992), S. 213-217 
    ISSN: 1432-0827
    Schlagwort(e): Porcine secretory enamel ; Degradation of amelogenin ; Proteinases ; 25kDa amelogenin ; Enzymography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary In the outermost layer of porcine-developing enamel adjacent to the ameloblasts in the secretory stage, the activities of two proteinases having molecular masses of 76 and 78kDa were detected by enzymography using gelatin as a substrate. On the other hand, high activities of known 30 and 34kDa proteinases were localized in the inner layer of the enamel. The 76kDa proteinase cleaved the carboxylterminal peptide of porcine 25kDa amelogenin to convert it to 20kDa amelogenin. The 78kDa proteinase also acted on the 25kDa amelogenin similarly, but its activity was weak. The results indicate that the 25kDa amelogenin synthesized and secreted by ameloblasts is converted to 20kDa amelogenin by the action of proteinase localized in the outermost layer of the secretory enamel, and then further degraded by the proteinases in the inner layer of the enamel associated with the increase of mineralization.
    Materialart: Digitale Medien
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 53 (1993), S. 257-261 
    ISSN: 1432-0827
    Schlagwort(e): Bovine enamelin ; Amelogenin ; Newly formed enamel ; Western blot
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary The possibility of using the antisera raised in rabbits against the porcine 25 kDa amelogenin, 32 and 89 kDa enamelins, and the 13–17 kDa nonamelogenin for the differentiation and identification of the protein components in bovine immature enamel was examined. Although the immunoreactivities of these antisera against bovine enamel proteins were weaker than those against the porcine proteins, it was found that these antisera could differentiate and demonstrate immunohistochemically a characteristic distribution of three different kinds of enamel protein components in the bovine secretory stage enamel similar to those observed in the porcine immature enamel. Of the several high molecular weight proteins being reactive to the anti-porcine 32 and 89 kDa enamelin sera, the 130 kDa protein, having the highest molecular weight, was extracted and purified from the bovine enamel sample which was obtained by peeling approximately 30-μm thickness of the outermost layer of the secretory stage enamel. The amino acid composition of the 130 kDa protein was similar to the known bovine enamelins, and was rich in aspartic acid, glutamic acid, proline, and glycine. The results could suggest that the enamelins of lower molecular weight than this protein, which are found in the bovine secretory stage enamel, are derived from this precursor protein.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 54 (1994), S. 69-75 
    ISSN: 1432-0827
    Schlagwort(e): Porcine secretory enamel ; Porcine amelogenin ; Plasma desorption mass spectometry ; Amino acid sequence ; CNBr cleavage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Abstract Amelogenins were extracted from the thin outer layer of porcine secretory enamel and purified by gel filtration and reverse-phase HPLC. The results of amino acid sequencing of the purified porcine amelogenins indicated the presence of at least four prototype amelogenins translated from alternatively spliced transcripts. The results of mass spectroscopy of the CNBr-cleaved peptides derived from the 25kDa amelogenin indicated that porcine 25kDa amelogenin is neither phosphorylated nor glycosylated.
    Materialart: Digitale Medien
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  • 17
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Enamel proteins were extracted from the newly formed layer of immature porcine enamel, and the 25 kDa amelogenin, 89 kDa enamelin and 13–17 kDa nonamelogenins were purified. Specific antisera were raised against these proteins. Antibodies specific to the C-terminal region (residues 149–173) of the 25 kDa amelogenin were generated by absorption of the anti-25 kDa amelogenin serum with 20 kDa amelogenin, which contains residues 1–148 of the antigen. Immunoelectrotransfer blotting of the extracted porcine enamel proteins showed that the anti-25 kDa amelogenin serum recognized the 25 kDa and other low and high molecular weight amelogenins. The C-terminal specific anti-25 kDa amelogenin serum reacted only with amelogenins having molecular weights over 23 kDa. The anti-89 kDa enamelin serum recognized the 89 kDa enamelin and lower molecular weight proteins, but neither the amelogenins nor the 13–17 kDa nonamelogenins. The antiserum against the 13–17 kDa nonamelogenins showed no cross reactivity to the 89 kDa enamelin, but recognized higher molecular weight nonamelogenins. In immunohistochemical preparations of the porcine tooth germs, the 25 kDa amelogenin-like immunoreactivity over immature enamel decreased in a gradient from the enamel surface to the middle layer. In the inner layer immunoreactivity was concentrated over the prism sheaths. The C-terminal specific 25 kDa amelogenin-like immunoreactivity was intense at the outer layer of immature enamel and decreased sharply toward the middle layer. Prism sheaths were intensely stained by the antiserum to the 13–17 kDa nonamelogenins. The 89 kDa enamelin-like immunoreactivity over enamel prisms was intense at the outer layer and decreased toward the middle layer. Staining by the anti-89 kDa enamelin serum of prism sheaths was faint. In immature rat incisor enamel, the C-terminal specific 25 kDa amelogenin antiserum demonstrated a staining pattern similar to that in the immature enamel of the pig. Distinct 13–17 kDa nonamelogenin-like and 89 kDa enamelin-like immunoreactivities were found especially in the layer adjacent to the Tomes' process. We conclude that some enamel proteins are degraded soon after their secretion from the secretory ameloblast in the rat and the pig. The specific enamel proteins which reacted with the antiserum to the 13–17 kDa nonamelogenins seem to be involved with the formation of prism sheaths in immature porcine enamel, but not in rat incisor enamel.
    Materialart: Digitale Medien
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  • 18
    ISSN: 1432-8798
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Antigenic variation of pseudorabies virus glycoprotein g II was demonstrated in field isolates by neutralizing monoclonal antibodies. Mutants capable of avoiding neutralization by the monoclonal antibodies specific for glycoproteins g II and g III could be obtained from the parent virus by mutagenesis.
    Materialart: Digitale Medien
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Mineralogy and petrology 46 (1992), S. 155-161 
    ISSN: 1438-1168
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Potosiit and Incait wurden in Erzen der Hoei-Mine, Oita Präfektur, Japan, nachgewiesen. Potosiit kommt in plattigen Kristallen (3 cm x 2 cm) vor and ist mit Arsenkies, Magnetkies, Zinnkies, Jamesonit and Pyrit assoziiert. Incait kommt in polykristallinen Aggregaten, die Ränder um Bleiglanz bilden, oder als Haar-artige Massen in einer Matrix von Kutnahorite vor. Röntgendiffraktionsdaten zeigen, daß es sich hier um Potosiit and Incait handelt; Mikrosondenanalysen der beiden Minerale geben die allgemeine Formel (Pb, Sn)2+ 6+x(FeSb2 4+)2S4+x, with x 〈 2. Potosiit ist homogen, während Incait in Kontakt mit Bleiglanz inhomogen ist. Quantitative Reflexionsdaten für Potosiit zeigen, daß seine Bireflexion zu den niedrigsten bei opaken Mineralen mit Schichtgitter gehört.
    Notizen: Summary Potosiite and incaite have been found at the Hoei mine, Oita Prefecture, Japan. Potosiite occurs as tabular crystals (3 cm x 2 cm in size), and is associated with arsenopyrite, pyrrhotite, stannite,jamesonite and pyrite, whereas incaite occurs as polycrystalline aggregates rimming galena or as hair-like masses in a matrix of kutnahorite. X-ray powder data reveal that these minerals are potosiite and incaite, respectively. Electron-microprobe analyses of potosiite and incaite gave the general formula (Pb, Sn)2+ 6+xFeSb2Sn4+ 2S14+x, with x 〈 2. Potosiite is homogeneous, whereas incaite, in contact with galena, is quite heterogeneous. Reflectance data for potosiite show that its bireflectance is one of the lowest among opaque minerals with a sheet-like structure.
    Materialart: Digitale Medien
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  • 20
    ISSN: 1619-7089
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
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