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  • 1
    Electronic Resource
    Electronic Resource
    Accuracy of the determination of S100B protein expression in malignant melanoma using polyclonal or monoclonal antibodies (2004)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 44 (2004), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims : To compare the routinely used polyclonal anti-S100 and a mouse monoclonal anti-S100B antibody for their accuracy in the detection of the S100B expression profile (pattern and intensity) in a series of 67 primary (n = 37) and lymph node metastatic (n = 30) melanoma tissues. S100B is the lineage marker of malignant melanoma. Antibodies routinely used for melanoma diagnosis are not necessarily specific for this protein. Furthermore, clinical monitoring of melanoma progression is mostly based on the determination of serum S100B protein levels without knowing the actual expression level in the primary and/or metastatic tissue.Methods and results : The profile of expression patterns (focal, heterogenous and diffuse) as well as intensity ranges (+, ++ and +++) were similar for the two antibodies in melanoma tissues. However, comparison of the patterns and intensities on the basis of individual cases revealed a high frequency of discrepancies (50.7 and 58.2%, respectively). Severe discrepancy between the two antibodies in the determination of the S100B protein expression pattern (focal versus diffuse or focal versus heterogeneous) was relatively frequent; 13.4 and 11.9%, respectively. Furthermore, a similar rate of severe discrepancy was observed between the two antibodies in the determination of the intensity of S100B expression levels (+ versus +++ or + versus ++); 19.4 and 8.9%, respectively. Separate analysis of the primary tumours and metastases gave similar results.Conclusion : For the accurate determination of S100B protein expression in malignant melanoma it is highly recommended that a monospecific antibody is used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2559.2004.01800.x
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  • 2
    Electronic Resource
    Electronic Resource
    Band structures in doubly-odd 100Rh (1998)
    Springer
    The European physical journal 2 (1998), S. 325-326 
    Details
    ISSN: 1434-601X
    Keywords: PACS:23.20.Lv Gamma transitions and level energies – 27.60.+j 90 〈 A 〈 149
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstact: High spin states of 100Rh have been populated using the reaction 70Zn+36S at 130 MeV. γ-rays were detected with the EUROGAM2 array. The level structure of 100Rh has been extended up to 14.41 MeV excitation energy. Several band structures are observed. A band based on a Iπ=8− state is developed up to the Iπ=24− level. It is assigned as the πg 9/2 −5νh 11/2 configuration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100500050126
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  • 3
    Electronic Resource
    Electronic Resource
    Experimental evidence for signature inversion in 132La from a revisited level scheme (2003)
    Springer
    The European physical journal 16 (2003), S. 1-5 
    Details
    ISSN: 1434-601X
    Keywords: PACS. 21.10.Hw Spin, parity, and isobaric spin – 21.10.Re Collective levels – 27.60.+j 90 ⩽ A ⩽ 149
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: The decay out of the πh 11/2νh 11/2 band to the known low-energy levels in 132La was studied using the reaction 100Mo + 36S at 160 MeV beam energy. The low-energy level scheme has been further developed and unambiguous spin and parity values have been assigned to the levels connecting the band to the 6- isomeric state. According to the new level scheme the spins in the πh 11/2νh 11/2 band are shifted up by one unit compared to the earlier tentative experimental values. The obtained new spins prove the existence of signature inversion in 132La and give further support to the spin assignments made for the πh 11/2νh 11/2 bands in the neighbouring odd-odd La isotopes from level energy systematics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1140/epja/i2002-10106-9
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  • 4
    Electronic Resource
    Electronic Resource
    Decay from superdeformed yrast states to normal deformed states in 193Tl (1998)
    Springer
    The European physical journal 2 (1998), S. 245-248 
    Details
    ISSN: 1434-601X
    Keywords: PACS:21.10.Re Collective levels – 23.20.Lv Gamma transitions and level energies – 27.80.+w 190 ≤ A ≤ 219
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Three high energy γ rays have been observed to connect the two yrast signature-partners superdeformed bands in 193Tl to the normal deformed states. Thereby, both excitation energy and spin of these bands are proposed for the first time in an odd-proton nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100500050115
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructure of invasion in different tissue types by lewis lung tumour variants (1990)
    Springer
    Virchows Archiv 417 (1990), S. 435-442 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural studies on the interactions of low and highly metastatic 3LL tumour lines with the basement membranes (BMs) of capillaries, veins, muscles, nerves and adipose tissue were performed by injecting tumour cells into the foot pad of mice. Haematogenous dissemination is the principle route of metastasis formation. Cells from the highly metastatic line were able to penetrate the blood vessels more efficiently than those from the low metastatic line. This difference was mainly due to a more pronounced diapedesis-like activity of the 3LL-HH cells, and partly to the altered intratumour vessel architecture in the highly metastatic tumour line. There was no difference between the two lines in the ultrastructure and frequency of invasion of nerves and adipose tissue BMs. However, in the highly metastatic line an extremely efficient penetration of muscle cell BM was observed. These results provide further evidence that the interaction of tumour cells with the BMs of different tissue types is one of the main determinants in local and distant dissemination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606032
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural localization and internalization of proteoglycan epitopes in a human non-Hodgkin (B) lymphoma (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 419-425 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a human non-Hodgkin (B) lymphoma xenograft (HT-117) heparan sulphate (HS) proved to be the main cell surface glycosaminoglycan, in contrast to the chondroitin sulphate dominance in normal lymphoid cells. Using anti-proteoglycan (PG) antibodies and immunoelectronmicroscopy, two heparan sulphate proteoglycans (transferrin receptor (TfR) and fibroblast membrane type) and one chondroitin sulphate proteoglycan (articular cartilage type) molecule were co-localized as random clusters on the surface of these lymphoma cells. Double labelling revealed that during internalization, which occurred via endosomes avoiding the lysosomal system, the different proteoglycan (PG) antigens became separated. The TfR and fibroblast membrane type HSPG epitopes reappeared on plasmalemmal vesicles derived most probably from the multivesicular endosomes, representing a unique form of exocytosis. It is suggested that different cell membrane PGs are integrated into subunits of yet unknown function in these human non-Hodgkin (B) lymphoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266450
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  • 7
    Electronic Resource
    Electronic Resource
    Two human melanoma xenografts with different metastatic capacity and glycosaminoglycan pattern (1989)
    Springer
    Journal of cancer research and clinical oncology 115 (1989), S. 554-557 
    Details
    ISSN: 1432-1335
    Keywords: Human melanoma ; Metastasis ; Glycosaminoglycan ; Xenograft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two human melanoma xenografts were compared with respect to their in vivo growth and metastatic potentials as well as glycosaminoglycan patterns. The less differentiated HT 168 tumor showed faster growth at primary sites and a more pronounced capacity for metastasis into the liver. Although chondroitin sulfate was the dominant glycosaminoglycan subtype in both tumors, the more invasive xenograft had a higher heparan sulfate/chondroitin sulfate (HS/CS) ratio. We suggest that tumor progression is influenced by this ratio in this human melanoma system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391357
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  • 8
    Electronic Resource
    Electronic Resource
    Interaction between elastin and tumor cell lines with different metastatic potential; in vitro and in vivo studies (1991)
    Springer
    Journal of cancer research and clinical oncology 117 (1991), S. 232-238 
    Details
    ISSN: 1432-1335
    Keywords: Elastin binding ; Elastin receptor ; Chemotaxis ; Ca2+ flux ; Lewis lung cell lines ; Cancer metastasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interactions between the extracellular matrix macromolecules and tumor cells are critical in the process of metastasis formation. We show here that elastins (both mature insoluble elastin and a 75-kDa soluble peptide: κ-elastin) adhere rapidly to two cell lines with high metastatic capacities: a metastatic lung carcinoma cell line (3LL-HM) and a human amelanotic melanoma cell line (A-2058); by contrast the low-metastatic Lewis lung carcinoma cell line variant as well as a rhabdomyosarcoma cell line with a low metastatic potential bind to elastins to a much lower extent.3H-labelled κ-elastin was used in order to study elastin-3LL-HM interaction. It was found to be saturable (2 ng3H-labelled κ-elastin/106 cells), with one class of high-affinity binding sites having Kd equal to 1.3 nM and 16000 sites/cell. The binding of κ-elastin to 3LL-HM cells at its receptor triggered several cell responses; (a) increase of intracellular Ca2+ concentration; (b) induction of 3LL-HM chemotaxis toward the κ-elastin gradient; (c) stimulation of the adherence of mature insoluble elastin. In contrast to non-transformed cells such as fibroblasts and smooth muscle cells, the adhesion kinetics of insoluble elastin to 3LL-HM did not exhibit a lag period; the rapid binding of insoluble elastin to the tumor cells was followed by its slow detachment from the cells, which lasted for 6 h. 3LL-HM cells but not human skin fibroblasts were shown to secrete elastinolytic activity inhibitable by metal-chelating agents. In vivo studies were performed in order to evaluate the influence of κ-elastin binding to 3LL-HM cells on their ability to form lung colonies in mice. It was shown that pretreatment of 104 3LL-HM cells with 10 ΜM kelastin and the simultaneous i.v. injection into mice of 750 Μg κ-elastin together with the highly metastatic cells was able to reduce the number of lung colonies by more than 70% after 12 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01625430
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  • 9
    Electronic Resource
    Electronic Resource
    Flow cytometric measurements and electron microscopy of cell surface glycosaminoglycans using Acridine Orange (1985)
    Springer
    Journal of molecular histology 17 (1985), S. 71-79 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell surface glycosaminoglycans (GAGs) were measured, after various treatments, by their binding to Acridine Organge using flow cytometry. Using a critical electrolyte concentration and combining it with specific degradation of individual GAG elements, it was found possible to differentiate between GAG components. The technique was adapted for electron microscopy level to reveal characteristics of membrane-associated GAG. By this means, the cell membrane of the human leukaemic cell line K562 was shown to contain a large amount of GAG; 75% of it was highly sulphated GAG, mostly heparan sulphate. This component was evenly distributed in the outer plasma membrane layer. In the presence of other GAGs, the appearance of complex proteoglycan granules was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01003404
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  • 10
    Electronic Resource
    Electronic Resource
    Experimental and clinicopathologic studies on the function of the HGF receptor in human colon cancer metastasis (2000)
    Springer
    Clinical & experimental metastasis 18 (2000), S. 639-649 
    Details
    ISSN: 1573-7276
    Keywords: c-met ; colon carcinoma ; in vitro ; metastasis ; motility ; progression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The organ-specific metastasis characterizes several human cancers, including colon carcinoma, a disease that frequently involves metastases in the liver. The data on the molecular mechanisms of liver metastasis would therefore be highly useful for prognostic purposes. Although the upregulation/amplification of the hepatocyte growth factor (HGF) receptor, c-met, has been frequently observed in colon cancer metastasis, the actual functional significance of the feature in the liver metastatization is not yet known. We have used three human colon carcinoma cell lines (HT29, HT25 and WiDr), characterized by different liver metastatic potentials in SCID mice, to analyze the expression of c-met and the biological effects of HGF. We found that HGF induces scattering in in vitro liver-metastatic cell lines (HT25 and WiDr) only at doses which are non-mitogenic (1–20 ng/ml). Analysis of the c-met expression revealed that the metastatic cell lines express authentic c-met gene and protein material, unlike the non-metastatic HT29 cell line, which expresses only the c-terminal cytoplasmic domain of the c-met β-chain. Interestingly, c-met was found to be localized in the substrate-attached peripheral membrane and partially colocalized with phosphotyrosine-proteins in the metastatic cells only when kept on fibronectin. On the other hand, we have analyzed 86 primary human colon cancers in Dukes' B (invasive but non-metastatic) and C (invasive and lymph node metastatic) stages. Western blotting of the proteins isolated from the tumor tissues and immunohistochemical control study on the paraffin samples of a third of these cases (25/86) all indicated a significant upregulation of the c-met protein in the Dukes' C tumor glands compared to the Dukes' B stages (P〈0.001 and P〈0.05, respectively). Since the two stages differ in the involvement of the regional lymph nodes but not in the invasion depth, the clinicopathological data and our experimental findings further support the notion that the c-met expression in human colon cancer can be considered as a marker of the metastatic potential due to its involvement in the generation of the motility signal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1013136303880
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