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  • 2000-2004  (1)
  • 1995-1999  (1)
  • Asymmetrically labelled fluorescence primer  (1)
  • PACS. 74.72.-h High Tc compounds – 74.25.Fy Transport properties (electric and thermal conductivity, thermoelectric effects, etc.)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Identification of mycobacteria to the species level by automated restriction enzyme fragment length polymorphism analysis (1995)
    Springer
    Virchows Archiv 427 (1995), S. 85-89 
    Details
    ISSN: 1432-2307
    Keywords: Automated laser fluorescence sequencer ; Partial digestion ; Restriction enzyme fragment length analysis ; Asymmetrically labelled fluorescence primer ; Mycobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An automated method for the restriction fragment length polymorphism (RFLP) analysis for the differentiation of mycobacteria to the species level is described. After polymerase chain reaction (PCR) amplification of a sequence of the gene encoding the 65-kDa surface antigen common to all mycobacteria the product was investigated by RFLP analysis. For accurate determination of fragment sizes the asymmetrically fluorescein-labelled PCR product was partially digested with restriction site enzymes BstEII and HaeIII. The fragments obtained were analysed electrophoretically using an automated laser fluorescence DNA sequencer. Determination of fragment sizes revealed a deviation of ±1 base pair (bp; 0.6%) when compared to expected sizes. The validity of this approach was confirmed by analysing mycobacterial DNA obtained from pure cultures of Mycobacterium (M.) tuberculosis and alcohol-fixed smears as well as paraffin-embedded sputa of patients with culture-proven tuberculosis. Additionally a diagnostic algorithm was established by investigation of cultured M. bovis, M. bovis bacille Calmette-Guérin, M. avium, M. intracellulare and M. fortuitum. The method allows the identification of restriction enzyme sites which are only 40 bp apart. Partial restriction enzyme digestion of asymmetrically fluorescence-labelled PCR products will presumably lead to the discovery of new restriction enzyme sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203742
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  • 2
    Electronic Resource
    Electronic Resource
    Thermal conductivity and thermal Hall effect in Bi- and Y-based high-T c superconductors (2001)
    Springer
    The European physical journal 20 (2001), S. 189-208 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 74.72.-h High Tc compounds – 74.25.Fy Transport properties (electric and thermal conductivity, thermoelectric effects, etc.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Measurements of the thermal conductivity (kxx) and the thermal Hall effect (kxy) in high magnetic fields in Y- and Bi-based high-T c superconductors are presented. We describe the experimental technique and test measurements on a simple metal (niobium). In the high-T c superconductors kxx and kxy increase below T c and show a maximum in their temperature dependence. kxx has contributions from phonons and quasiparticle (QP) excitations, whereas kxy is purely electronic. The strong increase of kxy below T c gives direct evidence for a strong enhancement of the QP contribution to the heat current and thus for a strong increase of the QP mean free path. Using kxy and the magnetic field dependence of kxx we separate the electronic thermal conductivity ( k xx el ) of the CuO 2 -planes from the phononic thermal conductivity ( k xx ph ). In YBa2Cu3O 7 - δ k xx el shows a pronounced maximum in the superconducting state. This maximum is much weaker in Bi2Sr2CaCu2O 8 + δ , due to stronger impurity scattering. The maximum of k xx el is strongly suppressed by a magnetic field, which we attribute to the scattering of QPs on vortices. An additional magnetic field independent contribution to the maximum of kxx occurs in YBa2Cu3O 7 - δ , reminiscent of the contribution of the CuO-chains, as determined from the anisotropy in untwined single crystals. Our data analysis reveals that below T c as in the normal state a transport (τ) and a Hall ( ) relaxation time must be distinguished: The inelastic (i.e. temperature dependent) contribution to τ is strongly enhanced in the superconducting state, whereas displays the same temperature dependence as above T c . We determine also the electronic thermal conductivity in the normal state from kxy and the electrical Hall angle. It shows an unusual linear increase with temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00011098
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    Paper (German National Licenses)
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