ISSN:
1432-2013
Keywords:
Electron microprobe analysis
;
Volume expansion
;
Intracellular electrolytes
;
Renal tubular cells
;
Natriuretic mechanisms
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract It has previously been shown that during mannitol-saline volume expansion (VE) Na transport was inhibited 50% by harvested proximal tubular fluid without a change in paracellular shunt pathway permeability to Na. To determine whether this inhibition was due to changes in cellular entry step or an effect on the pump itself, intracellular element concentrations were measured by electron microprobe X-ray ranalysis in proximal tubular cells of control (non-expanded, NE) and VE rats. Na i , Cl i and phosphorus i were increased (mean±S.E.) from 19.3±0.8 to 23.4±0.6, 15.8±0.4 to 21.3±0.4 and 124.3±2.6 to 138.0±1.8 mmol · kg−1 wet weight (P〈0.001) respectively while K i remained unchanged: 122.9±2.2 and 124.2±1.3 mmol · kg−1 wet weight. The increases in Na i and Cl i were in excess of cell shrinkage produced by the hyperosmolal peritubular environment while the unchanged K i in the face of cell shrinkage indicates and actual loss. It is concluded that mannitol-saline VE inhibits the Na pump producing a rise in Na i and a fall in K i .
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584101
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