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  • 2000-2004  (1)
  • 1980-1984  (3)
  • 1970-1974
  • Centrifugal elutriation  (2)
  • Arctic  (1)
  • Bacteriology of urine and renal calculus  (1)
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  • 2000-2004  (1)
  • 1980-1984  (3)
  • 1970-1974
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 12 (1984), S. 107-109 
    ISSN: 1434-0879
    Keywords: Infected urine ; Stone culture ; Bacteriology of urine and renal calculus ; Struvite calculus ; Oxalate calculus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The bacteriological status of the pre-operative urine, removed calculus and the pelvic urine was studied in 63 consecutive patients undergoing removal of intrarenal calculi. The overall infection rates in the pre-operative urine, removed calculi and pelvic urine were 29%, 38% and 30% respectively. In patients with staghorn calculi, 58% of patients had an infected pre-operative M.S.S.U., and 82.5% of removed calculi demonstrated significant bacterial growth, with Proteus mirabilis being the predominant organism isolated. In patients with single or multiple oxalate calculi, 17% had an infected pre-operative M.S.S.U., and 22% of removed calculi demonstrated a significant bacterial growth. The findings would indicate that the presence of a positive M.S.S.U. in a patient with a renal calculus is an indication for surgical removal.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0417
    Keywords: thecamoebians ; paleolimnology ; Holocene ; thermokarst lakes ; Arctic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Richards Island, Northwest Territories, Canada, is characterized by thermokarst lakes which record Holocene limnological change. This study is the first report of thecamoebian assemblages and continuous annual lake water temperatures from these Arctic lakes. Ecological environments on Richards Island are influenced by a climatic gradient resulting from the contrasting influences of the cold Beaufort Sea to the north and the warm waters of the Mackenzie Delta to the east and west. This climatic gradient in turn influences modern thecamoebian assemblages, and is an indication of the complexity involved in interpreting past conditions from core material in this area. Population abundance and species diversity of thecamoebian assemblages on Richards Island are not significantly different from those reported from temperate and semi-tropical latitudes. However, certain assemblage characteristics, such as large and coarse agglutinated tests, dominance of assemblages by one or two species and low morphological variation are interpreted to be diagnostic of Arctic conditions. Thecamoebian assemblages in core material from the area indicate that the local paleolimnological conditions may have changed within the last 3 ka, and this is unrecorded in previously reported pollen data. Paleoenvironmental interpretations in a permafrost landscape have to take into account morphological instability of thermokarst lakes, which can be the cause of paleolimnological and consequently faunal change. In this area ecosystem development is clearly related to geomorphology and local climatic effects and is not exclusively controlled by regional climate change.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2307
    Keywords: Mouse teratoma ; Centrifugal elutriation ; Neuroepithelial enrichment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Dissociation and centrifugal elutriation procedures were applied to subcutaneous transplants of the OTT-6050 mouse teratoma line in order to enrich the neuroepithelial cells. One of the resultant cell fractions, designated IB-21, was then implanted beneath the renal capsule of syngeneic mice and rebanked every 3 to 6 weeks for a total of 58 passages over 5 years. Sequential passages resulted in a tumor restricted to stem cells and neural cells (neuroblasts and glial cells). The primitive neural cells lost the ability to form rosettes after the early transplants. Subcutaneous or intracerebral transplantation of these tumors evinced their capacity for further neuroepithelial differentiation, with the demonstration of astrocytes and occasional mature synapse-forming neurons. Conversion of the tumor to the ascitic form resulted in unorganized clusters of neoplastic cells in contrast to the highly structured embryoid bodies that are characteristic of the parent OTT-6050 line. The absence of non-neural cells in the IB-21 tumor fraction and its ability to demonstrate divergent neural differentiation suggest that a transplantable neural-determined cell population exists in the OTT-6050 mouse teratoma.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2307
    Keywords: Mouse teratoma ; Centrifugal elutriation ; Melanotic neuroepithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Dissociation and elutriation procedures were applied to the OTT-6050 mouse teratoma line carried in subcutaneous implants in 129/J mice in order to enrich the differentiating neuroepithelial cells. Subsequent renal subcapsular implantation of one of the resultant cell fractions (IB-9) in syngeneic mice led to the constant production of macroscopically pigmented tumors which, in addition to undifferentiated stem cells, contained primitive neuroepithelial populations composed of medullary epithelium, neuroblasts, and numerous ependymoblastic rosettes. Melanin pigment, confirmed by the presence of melanosomes and premelanosomes, was found in medullary epithelium and other primitive neural cells. The tumors preserved their characteristics through 65 sequential transplants over a period of 5 1/3 years. The pigment was maintained in vitro for up to 3 months in an organ culture system. Subcutaneous or intracerebral transplantation of the renal tumors of the IB-9 fraction accentuated the capacity of these primitive cells towards further neuroepithelial differentiation into mature synapse-forming neurons, and was associated with a decrease in primitive neuroepithelium and an absence or a marked decrease of melanin. Return of the tumor to the kidney resulted in the reappearance of melanin after one to three passages, again associated with the presence of primitive neuroepithelium. The recognition of melanin pigment in the OTT-6050 mouse teratoma transplants could be a useful marker for the successful selection of primitive neuroepithelial cell populations in this experimental tumor system. These populations may help to study the relationship between melanin production and certain types of primitive neuroectodermal tumors in man.
    Type of Medium: Electronic Resource
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