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  • 2000-2004  (4)
  • 1975-1979  (5)
  • 1920-1924  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Connexin29 expression, immunocytochemistry and freeze-fracture replica immunogold labelling (FRIL) in sciatic nerve (2002)
    Oxford, UK : Blackwell Science, Ltd
    European journal of neuroscience 16 (2002), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The recently discovered connexin29 (Cx29) was reported to be present in the central and peripheral nervous systems (CNS and PNS), and its mRNA was found in particular abundance in peripheral nerve. The expression and localization of Cx29 protein in sciatic nerve were investigated using an antibody against Cx29. The antibody recognized Cx29 in HeLa cells transfected with Cx29 cDNA, while nontransfected HeLa cells were devoid of Cx29. Immunoblotting of sciatic nerve homogenate revealed monomeric and possibly higher molecular weight forms of Cx29. These were distinguished from connexin32 (Cx32), which also is expressed in peripheral nerve. Double immunofluorescence labelling for Cx29 and Cx32 revealed only partial colocalization of the two connexins, with codistribution at intermittent, conical-shaped striations along nerve fibers. By freeze-fracture replica immunogold labelling (FRIL), Cx32 was found in gap junctions in the outermost layers of myelin, whereas Cx29-immunogold labelling was found only in the innermost layer of myelin in close association with hexagonally arranged intramembrane particle (IMP) ‘rosettes’ and gap junction-like clusters of IMPs. Although both Cx32 and Cx29 were detected in myelin of normal mice, only Cx29 was present in Schwann cell membranes in Cx32 knockout mice. The results confirm that Cx29 is a second connexin expressed in Schwann cells of sciatic nerve. In addition, Cx29 is present in distinctive IMP arrays in the inner most layer of myelin, adjacent to internodal axonal plasma membranes, where this connexin may have previously unrecognized functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.2002.02149.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A brain slice model for in vitro analyses of astrocytic gap junction and connexin43 regulation: actions of ischemia, glutamate and elevated potassium (2000)
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 12 (2000), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Brain slices prepared from adult rats and maintained for up to 3 h in vitro were used to investigate the effects of pharmacological treatments on the phosphorylation state, immunolabelling characteristics and ultrastructural localization of astrocytic gap junctions and connexin43 (Cx43). Slices deprived of glucose/oxygen to mimic ischemia or those exposed to 1 mm glutamate for 1 h exhibited Cx43 dephosphorylation, epitope masking and gap junction internalization as revealed by Western blotting and Cx43 immunolocalization with various antibodies. Treatment with 15 mm K+ caused Cx43 dephosphorylation without junction internalization. The effects of glutamate and K+ were completely blocked by the N-methyl-d-aspartate (NMDA) glutamate receptor antagonist 2-amino-5-phosphonovalerate (APV), which acts largely on neuronal NMDA receptors, suggesting neuronal mediation of glial gap junction responses to these treatments. Astrocytes contained a dephosphorylated form of Cx43 with a typical migration profile at 41 kDa as well as novel, apparently dephosphorylated or partially phosphorylated, forms migrating at 43 kDa. These results indicate that slices prepared from adult brain can serve as a convenient model to investigate the molecular basis and receptor-mediated mechanisms underlying astrocytic Cx43 responses that have been observed in vivo following cerebral ischemia or neural activation. These processes can be related in part to neuronal regulation of astrocytic gap junctional coupling state, which is also amenable to analysis in brain slices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.2000.01331.x
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  • 3
    Electronic Resource
    Electronic Resource
    Connexin43 phosphorylation state and intercellular communication in cultured astrocytes following hypoxia and protein phosphatase inhibition (2000)
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 12 (2000), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effects of hypoxia and phosphatase inhibitors on connexin43 (Cx43) phosphorylation state, gap junctional intercellular communication (GJIC) and immunolabelling with anti-Cx43 antibodies were investigated in cultured astrocytes. Astrocytes contained predominantly phosphorylated forms of Cx43 and these underwent dephosphorylation 30 min after hypoxia. This was preceded by a 77% reduction in GJIC 15 min after hypoxia, indicating that reduced GJIC occurs prior to Cx43 dephosphorylation. Hypoxia caused a reduction in punctate immunostaining (epitope masking) at cell–cell contacts with one anti-Cx43 antibody, and increased labelling with another antibody (13–8300) that detects only a dephosphorylated form of Cx43. Inhibition of protein phosphatase (PP)-1 and PP-2A with okadaic acid or calyculin A had little effect on hypoxia-induced Cx43 dephosphorylation. Inhibition of PP-2B (calcineurin) with cyclosporin A or FK506 reduced Cx43 dephosphorylation and junctional uncoupling seen after hypoxia. These results demonstrate that responses of astrocytic Cx43 to hypoxia in vitro are similar to those seen after ischaemia in vivo, and that inhibition of protein phosphatase protects astrocytes from hypoxia-induced Cx43 dephosphorylation and junctional uncoupling. In addition, calcineurin may play a direct role in the regulation of astrocytic GJIC and Cx43 phosphorylation state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.2000.00162.x
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  • 4
    Electronic Resource
    Electronic Resource
    Direct evidence for presynaptic and postsynaptic dopamine receptors in brain (1978)
    [s.l.] : Nature Publishing Group
    Nature 274 (1978), S. 278-281 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Male Wistar rats (300-320 g) received unilateral 6-hyd-roxydopamine (6-OHDA) lesions of the NSP at the level of the lateral hypothalamus. After 25 d, the animals were killed and the striatum and SN were dissected out as previously described22. A small portion of the striatum was taken for tyrosine ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/274278a0
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Berichtigung zu meiner Arbeit: „Über Kurven vom Maximalklassenindex. Über Kurven vom Maximalindex”, Math. Ann. 89 (1923)
    Springer
    Mathematische Annalen 90 (1923), S. 150-151 
    Details
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01456247
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Thermooxidative investigation of silylated cellulose derivatives (1976)
    Springer
    Journal of thermal analysis and calorimetry 10 (1976), S. 59-64 
    Details
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Résumé La cellulose substituée par des groupes silico-organiques représente un nouveau type de celluloses modifiées. La présence du silicium change complètement les caractéristiques physiques et chimiques de la cellulose. L'étude thermo-analytique de divers dérivés de la cellulose triméthylsilylée a été effectuée dans l'air en atmosphère statique. Les courbes ATD de la cellulose utilisée comme matériau de départ sont différentes de celles de ses dérivés silylés. Pour ceux-ci, un nouveau pic exothermique caractéristique apparaît entre 320 et 370°C. La température du maximum du pic dépend du degré de la silylation et se déplace vers les valeurs plus élevées si le degré de substitution augmente.
    Abstract: Zusammenfassung Die durch Organosilikongruppen substituierte Zellulose stellt einen neuen Typ modifizierter Zellulose dar. Die Silylierung ändert die physikalischen und chemischen Eigenschaften der Zellulose drastisch. Die thermoanalytische Prüfung verschieden trimethylsilylierter Zellulosederivate wurde in Luft unter statischen Bedingungen durchgeführt. Die DTA-Kurven der als Ausgangsmaterial eingesetzten Zellulose sind von denen ihrer Silylderivate verschieden. In den letzteren erscheint ein neuer charakteristischer exothermer Peak im Bereich von 320 bis 370°C. Die Temperaturlage dieses Maximums hängt vom Silylierungsgrad ab und wird bei höherem Substitutionsgrad in Richtung höherer Temperaturen verschoben.
    Notes: Abstract Cellulose substituted by organosilicon groups represents a new type of modified cellulose. The silylation changes the physical and chemical properties of cellulose drastically. The thermoanalytical investigation of differently trimethylsilylated cellulose derivatives was carried out under static conditions in air atmosphere. The DTA curves of the cellulose used as starting material are different from those of its silylated derivatives. In the latter a new characteristic exothermic peak appears in the range 320–370°. The value of this maximum depends on the grade of silylation, being shifted towards higher temperatures with higher degrees of substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02179190
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  • 7
    Electronic Resource
    Electronic Resource
    Observation of site selective binding in a polymer nanotube composite (2000)
    Springer
    Journal of materials science 19 (2000), S. 2239-2241 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006776908183
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  • 8
    Electronic Resource
    Electronic Resource
    Über den Reaktionsmechanismus der Silylierung von Cellulose mit Hexamethyldisilazan in Pyridin (1978)
    Springer
    Colloid & polymer science 256 (1978), S. 290-291 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01550616
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  • 9
    Electronic Resource
    Electronic Resource
    Über den reaktionsmechanismus der silylierung von cellulose mit hexamethyldisilazan in pyridin (1976)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 177 (1976), S. 947-949 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1976.021770332
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  • 10
    Electronic Resource
    Electronic Resource
    1H and 13C studies of alkenes, epoxides and cyclic thionocarbonates (1977)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 10 (1977), S. 14-19 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 1H and 13C chemical shift data are presented and discussed for a number of di- and tri-alkyl substituted alkenes, epoxides and thionocarbonates. The completely stereospecific interconversion of these compounds, together with the n.m.r. data, allows a straightforward and quantitative stereochemical analysis. For 1H n.m.r., the most useful intermediates proved to be the thionocarbonates. The differential shieldings between cis and trans isomers are tentatively explained in terms of the conformational change of the 5-membered thionocarbonate ring. In 13C n.m.r., either series can be useful to distinguish between stereoisomers. The conclusions stemming from the 13C n.m.r. results complement the 1H n.m.r. studies.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270100105
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