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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 66 (2001), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : This study was undertaken to separate and characterize the porcine-specific thermostable muscle protein (psTSMP) recognized by monoclonal antibody, MAb 5H9, which was previously raised against crude porcine thermostable muscle proteins for detection of pork in cooked meats. The 24 kD antigenic components isolated from cooked pork by immunoaffinity chromatography consisted of a basic protein (pI = 9.4) and a group of isoelectric variants (pI ranging from 5.4 to 7.2). The basic protein was further isolated by methyl hydrophobic interaction and sulfonate ion-exchange chromatography. Immunoblot suggested the presence of this antigenic psTSMP in the tropo-nin fraction. Based on its molecular weight, pI, thermostability, and immunoreactivity, the psTSMP was tentatively identified as troponin I.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : :An indirect enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody (MAb) 2F8 specific to a mammalian muscle protein was developed to evaluate the endpoint heating temperature (EPT) in ground pork and beef. The ELISA response of MAb 2F8 with meat extracts increased rapidly as the EPT increased from 60 to 80 °C and leveled off above 80 °C. Results of immunoblot revealed that the 12 kD antigenic thermo-stable muscle protein, which is common in both pork and beef, can be used as an EPT indicator for heat-processed pork and beef based on changes in the ratio of this protein to total soluble proteins in the meat extract which occurs at the EPT.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Materials research innovations 3 (2000), S. 250-262 
    ISSN: 1433-075X
    Keywords: Key words Small-specimen testing ; Controlled-flaw method ; Fracture toughness ; Brittle materials ; Finite element analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: ∞ is the crack resistance at ”infinite” crack length. It is convincingly shown that this so-called R-curve equation correctly predicts K∞, which is comparable to the conventionally measured Mode I plain-strain fracture toughness, KIc, of the same material. The fundamental constants in the fracture-mechanics-based equations are discussed, emphasizing the aspects pertinent to the small specimens used in the MDBT. Results are presented on 8 materials: ZnS, glass-ceramic, Si3N4, Ti5Si3, SiC, Ni3Ge, NiAl and Ti-46.5A1-2.1Cr-3.0Nb-0.2W. All are brittle except for the latter two, which undergo slight plastic deformation before fracturing. The resulting values of K∞ are in excellent agreement with published values derived from conventional measurements, providing considerable confidence in the method. where Q is a constant and K∞ is the crack resistance at ”infinite” crack length. It is convincingly shown that this so-called R-curve equation correctly predicts K∞, which is comparable to the conventionally measured Mode I plain-strain fracture toughness, KIc, of the same material. The fundamental constants in the fracture-mechanics-based equations are discussed, emphasizing the aspects pertinent to the small specimens used in the MDBT. Results are presented on 8 materials: ZnS, glass-ceramic, Si3N4, Ti5Si3, SiC, Ni3Ge, NiAl and Ti-46.5A1-2.1Cr-3.0Nb-0.2W. All are brittle except for the latter two, which undergo slight plastic deformation before fracturing. The resulting values of K∞ are in excellent agreement with published values derived from conventional measurements, providing considerable confidence in the method.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0533
    Keywords: Protein Incorporation ; Amino Acids ; p-Bromophenylacetylurea ; Neuropathy ; Neurotoxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The incorporationin vivo of14C-glycine into proteins has been studied during the period before and after the onset of neurotoxicity caused by an organic mercury compound and byp-bromophenylacetylurea in rats. The observations of Yoshinoet al. (1966) on the former intoxication have been confirmed in that an impairment of glycine incorporation into proteins was present in spinal ganglion cells, and may also be taking place in other tissues, before nerve fibre degeneration takes place. A similar reduction in amino acid incorporation into proteins during the period before the onset of paralysis due top-bromophenylacetylurea is found in spinal ganglia; this finding is confirmed by anin vitro method using14C-leucine as protein precursor.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0533
    Keywords: Co-Radiation ; Spinal Cord ; Rat ; Cell Proliferation ; p-Bromophenylacetylurea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rats were irradiated with doses of 1 000–3 000 rads to the cervical spinal cord and subsequently given a paralytic dose of p-Bromophenylacetylurea. The nuclear populations in the degenerating dorsal columns were determined and it was found that a significant suppression of cell proliferation occurred after all three dose levels. The cell populations in the shielded parts of the tracts rostral to the irradiated zone were not affected.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 19 (1971), S. 208-215 
    ISSN: 1432-0533
    Keywords: Organic Mercury ; Neuropathy ; Toxicology ; Neuronal ; Degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rats have been poisoned with methyl-mercury-dicyandiamide at two different dose levels, 5 mg/kg body weight or 7.5 mg/kg daily for 8 consecutive days. The morphological changes in peripheral nerves and the central nervous system are largely restricted to primary sensory cells of spinal ganglia, and to a lesser extent to the granular cells of the cerebellum. There were clearly two grades of cell damage, either whole cell death or whole fibre death; there is no evidence to suggest that partial fibre death could occur, at least not at the two dose levels used. No definite indications were found for this neuropathy being a ‘dying back’ process, for the nerve fibres appeared to degenerate contemporaneously along their whole extent.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 16 (1972), S. 2175-2184 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Capillary flow data were obtained for a 27.5% solution of cellulose acetate in acetone. The solution temperature was 50°C, and the range of apparent shear rates investigated was 1.7 × 105 to 1.7 × 106 sec-1. Capillaries having tapered entrance angles of 37.88° to 120.63° were used. A power-law model was adequate to describe the shear stress at the wall (τw) and the corrected shear rate \documentclass{article}\pagestyle{empty}\begin{document}$(\dot \gamma )$\end{document} relationship. Entrance angle affected the entrance pressure drop corrected for kinetic energy, (ΔP0,c); ΔP0,c increased as the angle widened. Treating the entrance flow as an elongational flow situation facilitated superposition of the Delta;P0,c data on a single curve. Estimated elongational viscosities decreased with increasing applied stress.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The angular distribution of scattered intensity and decay times of concentration fluctuations have been measured by means of digital photon counting and single-clipped photon correlation for solutions of Group C meningococcal polysaccharides at 31°C. The z-average diffusion coefficient 〈D〉z and its second moment 〈D2〉z have been determined from the time-dependent correlation function using the cumulant expansion technique. Very low observed values of 〈D〉z and the tremendous width of the polydispersity index, which is the z-average normalized variance, suggest a higher degree of aggregation than the monomer-dimer type self-association at finite concentrations.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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