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  • 2000-2004  (4)
  • 1955-1959  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Quantitative analysis of UVB-induced apoptosis in human epidermis (2000)
    Copenhagen : Munksgaard International Publishers
    Experimental dermatology 9 (2000), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The quantitative measurement of the induction of apoptosis in cells grown in vitro can be accomplished using a variety of proven methods. However, the quantitative assay of apoptosis within an intact tissue is very laborious and the results can be misleading. We have established a method to quantitatively analyze the induction of apoptosis in human epidermis following UVB irradiation. The assay is based on the activation of the apoptotically induced enzyme caspase 3, using a synthetic caspase 3 substrate. The activation of caspase 3 was shown to correlate with the induction of apoptosis in human keratinocytes cultures as a monolayer. We then demonstrated that the activation of caspase 3 could be measured from UVB-irradiated whole skin. The induction of apoptosis was confirmed by cellular morphology and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling. Therefore, we concluded that the measurement of caspase 3 specific activity in UVB-irradiated human epidermis was an efficient, inexpensive, and accurate method to quantitate UVB-induced apoptosis in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0625.2000.009003185.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Use of enhanced green fluorescent protein to monitor retroviral-mediated gene therapy in human keratinocytes (2000)
    Copenhagen : Munksgaard International Publishers
    Experimental dermatology 9 (2000), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Keratinocytes have great promise as targets for gene therapy involving both skin as well as for systemic disorders due to their availability and potential long life span. Improvement of gene transfer into keratinocytes will be greatly facilitated by markers that will allow both rapid detection and efficient selection of transduced cells. For these purposes, a recombinant version of the Aequorea victoria green fluorescent protein that is enhanced for high-level expression in mammalian cells (EGFP) was placed into a replication-deficient retroviral vector. High-titer retrovirus was used to transduce both primary cultures of neonatal foreskin-derived human keratinocytes (HK) as well as the immortalized keratinocyte-derived cell line HaCaT. Both cell types stably expressed the EGFP, and this marker allowed rapid purification of transduced cells by fluorescence-activated cell sorting. EGFP expression was seen in HaCaT keratinocytes for at least 40 passages, and the presence of this construct did not effect cell growth, or apoptosis in response to UVB or etoposide. Transduced populations of HK were grafted into SCID mice, resulting in a functional epidermis. EGFP expression was readily seen in vivo by exposing the xenografts to an ultraviolet light source. These studies demonstrate the feasibility of using EGFP as a convenient and rapid marker to monitor keratinocyte gene transfer both in vitro and in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0625.2000.009004252.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of Age on Serum Dehydroepiandrosterone Sulfate, IGF-I, and IL-6 Levels in Women (2000)
    Springer
    Calcified tissue international 66 (2000), S. 414-418 
    Details
    ISSN: 1432-0827
    Keywords: Key words: DHEAS — IGF-I — IL-6 — Age.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Data from animal and in vitro studies suggest that the growth-promoting effects of the adrenal androgen dehydroepiandrosterone sulfate (DHEAS) may be mediated by stimulation of insulin-like growth factor-I (IGF-I) and/or inhibition of interleukin 6 (IL-6), a cytokine mediator of bone resorption. This study tests the hypotheses that there are effects of age on serum DHEAS, IGF-I, and IL-6 levels, and that levels of IGF-I and IL-6 are related to DHEAS levels. The study included 102 women: 27 premenopausal and 75 postmenopausal, including 35 postmenopausal women with osteoporosis, as defined by bone mineral density scores by dual X-ray energy absorptiometry. DHEAS levels decreased significantly with age (r =−0.52, P 〈 0.0001) and IGF-I levels decreased significantly with age (r =−0.49, P 〈 0.0001). IL-6 levels increased significantly with age (r = 0.36, P= 0.008). IGF-I was positively correlated to DHEAS levels (r = 0.43, P 〈 0.0001, n = 102) and IL-6 levels were negatively correlated to DHEAS levels (r =−0.32, P= 0.021, n = 54). Levels of DHEAS and IGF-I were correlated with T scores of the spine and some hip sites. In a multiple variable model to predict DHEAS, age was an important predictor (P 〈 0.001), but osteoporosis status, IGF-I, and IL-6 were not. The median DHEAS level was lower in the postmenopausal osteoporotic women (67 μg/dl, n = 35) than in the nonosteoporotic postmenopausal women (106.3 μg/dl, n = 40, P= 0.03), but this was not significant after correction for age. Age accounted for 32% of the variance in DHEAS levels. In summary, DHEAS levels decreased with age and had a positive association with IGF-I levels and a negative association with IL-6 levels. DHEA deficiency may contribute to age-related bone loss through anabolic (IGF-I) and anti-osteolytic (IL-6) mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002230010084
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Association of posterior tibial tendon abnormalities with abnormal signal intensity in the sinus tarsi on MR imaging (2000)
    Springer
    Skeletal radiology 29 (2000), S. 514-519 
    Details
    ISSN: 1432-2161
    Keywords: Key words Ankle ; Foot ; Tendon ; Sinus tarsi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective. To evaluate the association of abnormal signal intensity within the sinus tarsi with abnormalities of the posterior tibial tendon (PTT) on MR imaging. Design and patients. Sinus tarsi abnormalities were identified on 30 ankle MR examinations in 29 patients. The PTT and anterior talofibular ligament were retrospectively analyzed for abnormalities in these same patients. Results and conclusions. Tears of the anterior talofibular ligament were found in 13 of 30 (43%) ankles. PTT abnormalities (complete tear, partial tear or dislocation) were seen in 14 of 30 (47%) studies, and were distributed relatively equally between those patients with and without lateral ligament tears. Our results provide evidence of an association between abnormalities of the PTT and the sinus tarsi. The finding of abnormal signal intensity within the sinus tarsi on MR imaging should alert the radiologist to potential abnormalities of the PTT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560000248
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Fertilizing value of “Huleh” peat (1956)
    Springer
    Plant foods for human nutrition 2 (1956), S. 1-19 
    Details
    ISSN: 1573-9104
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary 1. Pot- and field-experiments were carried out in both sandy and medium clay soils to determine the fertilizing value of Huleh peat, both alone and in combination with manure and/or KPN. 2. Tests to compare the value of light and heavy peat-treatments were carried out in both pot and field-experiments. 3. Tests to determine the relative effectiveness of peat from upper and lower layers (0.5, 1.0 and 3.0 m) and from various locations, were carried out in the pot-experiments. 4. Persistence of fertilizing-value of peat was compared with that of manure in the pot-experiments. 5. Pot-experiments with dehydrated peat were carried out to determine the best drying method from the standpoint of fertilizing-value. 6. Preliminary trials were made to determine the most effective method of combining peat and KPN. 7. Botanical and soil-survey data is briefly reviewed and a chemical analysis of the peat is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01889768
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    Paper (German National Licenses)
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