ISSN:
1437-1596
Keywords:
Key words Sudden infant death syndrome (SIDS)
;
Fetal
;
haemoglobin
;
Rat globin
;
High performance liquid chromatography (HPLC)
;
Extramedullary haematopoiesis (EMH)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Law
Notes:
Abstract The influence of repeated hypoxia on the development of haemoglobin (Hb) subtypes and on extramedullary haematopoiesis (EMH) was investigated in young Wistar rats of different ages. The rats were exposed to hypercapnic/hypoxic and to “simple” hypoxic conditions. The results obtained were compared to those of an untreated age-matched control group. Different globin chains were measured using HPLC and time-of-flight (TOF) mass analysis. The number of EMH cells was evaluated by cell counting. By determining the proportions of α- and β-chains, fetal, neonatal and mature types of globin chain composition could be differentiated. The β-2 chain levels were significantly higher in hypercapnic/hypoxic environments than in the controls and simple hypoxic environments. The numbers of EMH cells in the two groups subjected to hypercapnia/hypoxia decreased significantly more slowly compared to the controls and simple hypoxia groups. Therefore, the development of Hb subtypes and the EMH activity in rats were influenced by both repeated hypercapnia and hypoxia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004140000151
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