ISSN:
1432-2242
Keywords:
Key words Wheat transformation
;
Ribosome-inactivating protein
;
Anti-fungal protein
;
Protein-targeting
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed ß-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051349
Permalink
