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  • 1
    Electronic Resource
    Electronic Resource
    Involvement of nucleocapsids in reverse transcription: a general phenomenon?
    Amsterdam : Elsevier
    Trends in Biochemical Sciences 12 (1987), S. 92-95 
    Details
    ISSN: 0968-0004
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0968-0004(87)90044-2
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cauliflower mosaic virus as a gene expression vector for plants (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: It is possible to replace the CaMV (cauliflower mosaic virus) ORF (open reading frame) II with foreign sequences without interfering with virus viability. Such recom-binants can induce the synthesis of substantial amounts of a foreign protein in infected plants and confer new properties to these plants. However, so far only three genes have been successfully cloned and expressed in this way. The expression mechanism of CaMV demands precise replacement of ORF II and probably certain structural features of the viral 35S RNA, which should not be disturbed by inserted sequences. Since these features are largely unknown, it cannot at present be pre-dicted whether an insert will be tolerated. It is more likely that larger inserts will disturb the viral gene expression mechanism than smaller ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05878.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic engineering of wheat for increased resistance to powdery mildew disease (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1079-1086 
    Details
    ISSN: 1432-2242
    Keywords: Key words Antifungal proteins ; Chitinase ; Biolistic transformation ; Wheat ; Chlorophenolred ; Phosphinotricin ; Erysiphe graminis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Fungal wheat (Triticum aestivum) diseases greatly affect crop productivity and require the economically and ecologically undesirable application of fungicides in wheat agriculture. We have generated transgenic wheat plants constitutively expressing an antifungal barley-seed class II chitinase. The transgene was stably expressed and the chitinase properly localized in the apoplast of the transgenic lines. The engineered wheat plants showed increased resistance to infection with the powdery mildew-causing fungus Erysiphe graminis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051170
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of active barley seed ribosome-inactivating protein in transgenic wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 755-763 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat transformation ; Ribosome-inactivating protein ; Anti-fungal protein ; Protein-targeting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed ß-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051349
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic engineering of Indica rice in support of sustained production of affordable and high quality food in developing countries (1955)
    Springer
    Euphytica 85 (1955), S. 441-449 
    Details
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Indica-type rice ; genetic engineering ; vitamin A endosperm ; insect resistance ; virus resistance ; fungus resistance ; essential amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Indica-type rice provides the staple food for two billion people in Third World countries. Several problems involved in the stable and sustained production of high quality food cannot be solved by traditional breeding. Methods have been established for gene transfer to Indica rice breeding lines to study possible contributions from genetic engineering. Experiments are in progress on the development of transgenic resistance towards Yellow Stem Borer, resistance towards Rice Tungro Virus, accumulation of provitamin A in the endosperm, increase of essential amino acids in the endosperm such as lysine, cysteine and methionine and resistance towards fungal pests such as Rice Blast and Sheath Blight. Transgenic clones from Indica rice breeding lines have been recovered from several of the approaches mentioned, some of which have been regenerated to plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023978
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