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Hydrogen peroxide induces association between glyceraldehyde 3-phosphate dehydrogenase and phospholipase D2 to facilitate phospholipase D2 activation in PC12 cells (2003)

Kim, Jung Hwan ; Lee, Sukmook ; Park, Jong Bae ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 85 (2003), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Oxidative stress or signaling is widely implicated in apoptosis, ischemia and mitogenesis. Previously, our group reported that the hydrogen peroxide (H2O2)-dependent activation of phospholipase D2 (PLD2) in PC12 cells is involved in anti-apoptotic effect. However, the precise mechanism of PLD2 activation by H2O2 was not revealed. To find H2O2-dependent PLD2-regulating proteins, we immunoprecipitated PLD2 from PC12 cells and found that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) coimmunoprecipitated with PLD2 upon H2O2 treatment. This interaction was found to be direct by in vitro reconstitution of purified GAPDH and PLD2. In vitro studies also indicated that PLD2-associated GAPDH was modified on its reactive cysteine residues. Koningic acid, an alkylator of GAPDH on catalytic cysteine residue, also increased interaction between the two proteins in vitro and enhanced PLD2 activity in PC12 cells. Blocking H2O2-dependent modification of GAPDH with 3-aminobenzamide resulted in the inhibition of the GAPDH/PLD2 interaction and attenuated H2O2-induced PLD2 activation in PC12 cells. From the results, we suggest that H2O2 modifies GAPDH on its catalytic cysteine residue not only to inactivate the dehydrogenase activity of GAPDH but also to endow GAPDH with the ability to bind PLD2 and the resulting association is involved in the regulation of PLD2 activity by H2O2.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1471-4159.2003.01755.x

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Efflorescence and Leaching Behavior of Metal Ions for EAF Dust-Clay Based Bricks (2004)

Kim, Jung Hwan ; Lee, Ki Gang ; Kim, Y. ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Materials science forum Vol. 449-452 (Mar. 2004), p. 241-244

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ISSN: 1662-9752

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Brick samples were prepared through vitrification process with two different kinds of EAF dust. Leaching concentrations of various metal ions and anions for the bricks(20wt% EAF dust-80wt% clay) were evaluated by ICP and IC analysis. The results of leaching test showed that the concentration of ions leached from bricks were proportional to the square root of time and saturated with leaching time. The saturated concentrations of alkali and alkali-earth metal ions were 0.1-250ppm; on the other hand, the heavy metal ions were 0-0.084ppm. The efflorescence mechanism of EAF dust-clay bricks was studied by quantitative analysis for the effects of various metal ions and anions. Efflorescence formed on the brick surfaces which were immersed in DI water and dried slowly in the air were characterized by SEM, EDS and XRD. The results of EDS and XRD showed that the main componentof the efflorescence was water soluble Na〈sub〉2〈/sub〉SO〈sub〉4〈/sub〉