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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Oxidative stress or signaling is widely implicated in apoptosis, ischemia and mitogenesis. Previously, our group reported that the hydrogen peroxide (H2O2)-dependent activation of phospholipase D2 (PLD2) in PC12 cells is involved in anti-apoptotic effect. However, the precise mechanism of PLD2 activation by H2O2 was not revealed. To find H2O2-dependent PLD2-regulating proteins, we immunoprecipitated PLD2 from PC12 cells and found that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) coimmunoprecipitated with PLD2 upon H2O2 treatment. This interaction was found to be direct by in vitro reconstitution of purified GAPDH and PLD2. In vitro studies also indicated that PLD2-associated GAPDH was modified on its reactive cysteine residues. Koningic acid, an alkylator of GAPDH on catalytic cysteine residue, also increased interaction between the two proteins in vitro and enhanced PLD2 activity in PC12 cells. Blocking H2O2-dependent modification of GAPDH with 3-aminobenzamide resulted in the inhibition of the GAPDH/PLD2 interaction and attenuated H2O2-induced PLD2 activation in PC12 cells. From the results, we suggest that H2O2 modifies GAPDH on its catalytic cysteine residue not only to inactivate the dehydrogenase activity of GAPDH but also to endow GAPDH with the ability to bind PLD2 and the resulting association is involved in the regulation of PLD2 activity by H2O2.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 449-452 (Mar. 2004), p. 241-244 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Brick samples were prepared through vitrification process with two different kinds of EAF dust. Leaching concentrations of various metal ions and anions for the bricks(20wt% EAF dust-80wt% clay) were evaluated by ICP and IC analysis. The results of leaching test showed that the concentration of ions leached from bricks were proportional to the square root of time and saturated with leaching time. The saturated concentrations of alkali and alkali-earth metal ions were 0.1-250ppm; on the other hand, the heavy metal ions were 0-0.084ppm. The efflorescence mechanism of EAF dust-clay bricks was studied by quantitative analysis for the effects of various metal ions and anions. Efflorescence formed on the brick surfaces which were immersed in DI water and dried slowly in the air were characterized by SEM, EDS and XRD. The results of EDS and XRD showed that the main componentof the efflorescence was water soluble Na〈sub〉2〈/sub〉SO〈sub〉4〈/sub〉
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 124-126 (June 2007), p. 1585-1588 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Notes: The corrosion behavior of glass and glass-ceramics fabricated with silicate glass frit mixedwith 50~70 wt% EAF dust in the acidic solution was analyzed by both heavy metal leaching test andmicrostructural observation. The crystallization temperature, Tc of glassy specimens was around 850[removed info] measured by DTA and the heat treatment temperature to crystallize a glassy specimen was selectedas 950 [removed info]/ 1 hr. The spinel crystal peaks were found in XRD analysis for the glass containing dust 〉60 wt%. For the glass-ceramics, however, the spinel peaks in a specimen containing dust 〉 50 wt%was found with weak willemite peaks. The glass and glass-ceramic specimens showed the first stageof corroding reaction according to Clark models in acidic solution. The glass-ceramic specimensshowed much lower a heavy metal leaching concentration than that of glass specimens in thecorrosion test in acidic solution of pH=2.95. Especially, the glass-ceramics containing dust 60 wt%showed a heavy metal leaching concentration of 66 % Pb, 60 % Zn and 98 % Fe lower than that ofglass specimens due to crystal phases formed, thermodynamically more stable than a glass networkstructure. From the leaching test that more Zn ion leached out than Fe ion, the spinel crystal phase[ZnFe2O4] showed better corrosion resistant in the acidic solution than the willemite [Zn2SiO4]
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 486-487 (June 2005), p. 382-386 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Stabilizing characteristics of heavy metals in the silicate glass (SD) and lead silicate glass(PD) containing Electric Arc Furnace(EAF) dust was studied by the toxic characterization leaching procedure(TCLP) test. Dependence of the amount of EAF dust upon structural changes of SD and PD glasses and TCLP results was also investigated by FT-IR spectroscopy. In the TCLP test,the concentration of heavy metals leached from a glass increased with the amount of EAF dust added. The SD series specimens showed heavy metal leaching lower than the PD series specimens, but the Pb leaching from the PD series specimens was the highest owing to the high Pb content in the PD glass composition. The value R(oxygen/network former ion ratio) was used to compare the leachingcharacteristics of glasses, and the SD had a higer R than PD series specimens to show a better chemical durability. Adding the EAF dust to the SD mother glass decreased the Si-O-Si symmetry and increased the non-bridging oxygen, which weakened the structure and decreased the chemical durability of glasses. It is concluded that SD is more effective in stabilizing the heavy metals of EAFdust than PD series glass
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 486-487 (June 2005), p. 105-108 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Thermal behaviors of EAF dust, water-washed EAF dust, and EAF dust/heavy claymixtures with wet mixing process were characterized by TG/ DTA analysis. The ionic concentrations of some metal ions and anions in the supernatant from water-washing process were evaluated by ICP and IC analysis. Weight loss of water-washed EAF dust above the temperature range of 1000℃ was significantly decreased because of the washing effect of chloride and sulfate compounds. Thevariation of volatile ZnO and PbO contents were not detected in 20wt% D dust-80wt% clay sample. The wet mixing process not only enhanced the homogeneous mixing of EAF dust and clay particles, but also reduced the volatilization of heavy metals(Zn, Pb, etc.) at the high temperature range
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 544-545 (May 2007), p. 585-588 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Plasticity indexes (PI) of clay bodies including bottom ashes (BA) and various wastes byusing Atterberg limits were measured for the effective recycling of coal bottom ashes from thepower plant. Coal bottom ash (BA)-red clay (RC or simply clay), dredged soil (DS)-BA, coal flyash (FA)-red clay, DS-FA were used as samples for the PI measurement. From the comparison ofclay bodies with BA and DS, PI of DS-BA body was higher than that of BA-red clay body becauseliquid limit (LL) was high and plastic limit (PL) of DS-red clay was low, respectively. Comparingclay bodies with FA and BA, PI of BA-clay body was higher than that of FA-clay body becauseboth LL and PL of BA-clay body were low. When stone ashes and sewage sludge (SS) were addedto BA-clay bodies, PI decreased with increasing both stone ash and sewage sludge contents. It isconcluded that the measurement of plasticity index can be used as an indicator of formingperformance of green bodies with various compositions
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 544-545 (May 2007), p. 601-604 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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